Cryptosporidium ELISA Kits are designed for the detection and quantification of Cryptosporidium species in biological samples. Cryptosporidium is a genus of protozoan parasites that cause cryptosporidiosis, a disease that can lead to gastrointestinal symptoms such as diarrhea. These kits are commonly used in research and diagnostic laboratories to identify and measure Cryptosporidium in various samples.
Content of the Kit
- Pre-coated 96-Well Microplate:
- Wells pre-coated with an antibody specific to Cryptosporidium antigens, allowing for the capture of Cryptosporidium from the samples.
- Standards:
- Solutions containing known concentrations of Cryptosporidium antigens, used to generate a standard curve for quantifying Cryptosporidium levels in samples.
- Sample Diluent:
- A buffer solution used to dilute biological samples or environmental samples to appropriate concentrations for accurate measurement.
- Detection Antibody:
- An antibody specific to Cryptosporidium antigens, often conjugated to an enzyme such as HRP for detection.
Procedure
- Coating:
- Coat the wells of the microplate with an antibody specific to Cryptosporidium antigens. Incubate to allow binding and then wash away unbound antibody.
- Blocking:
- Block any remaining uncoated surfaces in the wells with a blocking buffer to prevent non-specific binding.
- Addition of Standards and Samples:
- Add standards and samples to the wells. Cryptosporidium antigens in the samples will bind to the immobilized antibody.
- Incubation:
- Incubate the plate to allow Cryptosporidium antigens to bind to the antibody.
- Addition of Detection Antibody:
- Add a detection antibody specific to Cryptosporidium antigens, which binds to the captured Cryptosporidium.
Performance Characteristics
- Sensitivity: Capable of detecting low levels of Cryptosporidium, often in the range of picograms to nanograms per milliliter (pg/mL to ng/mL).
- Precision: Shows low intra-assay and inter-assay variability, with coefficients of variation (CV) generally below 10%.
- Linearity: Demonstrates linearity across the standard curve range, ensuring accurate quantification.
- Recovery: High recovery rates, usually above 85%, indicating reliable performance across different sample types.
Applications
- Clinical Diagnostics: Detecting Cryptosporidium infections in patients with gastrointestinal symptoms.
- Epidemiological Studies: Monitoring the prevalence and distribution of Cryptosporidium infections in populations.
- Environmental Monitoring: Testing water and environmental samples for Cryptosporidium contamination.
- Research: Investigating the pathogenesis of Cryptosporidium and developing new treatment and prevention strategies.
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