CSN ELISA Kits

CSN (COP9 Signalosome) ELISA kits are specialized immunoassays designed for the quantitative detection of CSN complex proteins in biological samples such as serum, plasma, and cell or tissue lysates. The COP9 signalosome is a multi-protein complex that plays a critical role in cellular processes such as protein degradation, signal transduction, and cell cycle regulation. It regulates the activity of E3 ubiquitin ligases and is involved in the ubiquitin-proteasome system, impacting various cellular pathways linked to development, cancer, and immune responses.

Content of CSN ELISA Kits: CSN ELISA kits typically include:

• A 96-well microplate pre-coated with specific capture antibodies targeting one or more subunits of the CSN complex (e.g., CSN5).
• Recombinant CSN protein standards for creating a standard curve.
• Detection antibodies conjugated with an enzyme such as HRP (Horseradish Peroxidase) for signal development.
• Substrate solution (e.g., TMB – 3,3',5,5'-Tetramethylbenzidine) for colorimetric detection.
• Stop solution to halt the enzymatic reaction.
• Sample and assay diluents, wash buffers, and a comprehensive protocol for sample preparation and assay execution.

Application of CSN ELISA Kits: CSN ELISA kits are utilized in a range of research and clinical applications, including:

• Cancer research: Studying the role of the CSN complex in tumor development, as it is implicated in the regulation of cell proliferation, apoptosis, and DNA damage responses. Dysregulation of CSN subunits can contribute to oncogenesis.
• Cell cycle and growth regulation: Measuring CSN protein levels to understand its impact on the cell cycle and how it influences cell growth and division.
• Proteasome function studies: Investigating how the CSN complex regulates the ubiquitin-proteasome system, contributing to the targeted degradation of proteins and maintaining cellular homeostasis.
• Signal transduction research: Analyzing the involvement of CSN in various signaling pathways that impact cellular responses to environmental cues.
• Developmental biology: Exploring CSN’s role in embryonic development and differentiation due to its regulatory functions in protein modification and degradation.
• Immunology: Studying CSN's contribution to immune cell function and its role in immune response modulation.

CSN ELISA kits are valuable tools for researchers aiming to understand the complex roles of the COP9 signalosome in cellular processes and disease mechanisms, offering essential insights for studies in cancer biology, cell cycle regulation, and protein homeostasis.