Overview of CSP ELISA Kits

CSP ELISA Kits are designed for the quantitative measurement of Circadian Locomotor Output Cycles Protein Kaput (CLOCK) in various biological samples. CLOCK proteins are essential components of the circadian clock, regulating various physiological processes including sleep-wake cycles, hormone release, and metabolism.

Technical Content

Key Features:

• High Sensitivity: Capable of detecting low levels of CLOCK proteins, ensuring precise quantification even in samples with low expression.
• Specificity: High specificity for CLOCK proteins, minimizing cross-reactivity with other proteins.
• Wide Range of Applications: Suitable for use with serum, plasma, cell lysates, and other biological fluids.

Applications:

• Chronobiology Research: Used to study the mechanisms of circadian rhythms and the regulation of biological clocks.
• Sleep Research: Facilitates the investigation of CLOCK proteins' roles in sleep disorders and the effects of sleep disruption.
• Metabolic Studies: Assists in understanding the involvement of CLOCK proteins in metabolic pathways and diseases such as diabetes and obesity.

Usage Protocol:

• Sample Preparation: Collect and prepare samples according to the kit instructions. Dilute if necessary.
• Assay Procedure: Add samples, standards, and controls to the wells of the ELISA plate pre-coated with antibodies specific to the target CLOCK protein.
• Incubation: Incubate the plate to allow binding of the CLOCK proteins to the antibodies.
• Detection: Add detection antibodies and enzyme substrate to produce a colorimetric signal proportional to the CLOCK protein concentration.
• Analysis: Measure the absorbance using a microplate reader and calculate CLOCK protein concentrations based on the standard curve.

Benefits:

• Precision: Provides reliable and reproducible results for the accurate quantification of CLOCK proteins.
• Efficiency: Simplified protocol with ready-to-use reagents reduces assay time and labor.
• Versatility: Compatible with a variety of sample types, making it suitable for diverse research applications.