CTLA ELISA Kits

CTLA (Cytotoxic T-Lymphocyte Antigen) ELISA kits are highly specialized diagnostic tools designed for the quantitative detection and analysis of CTLA proteins, specifically CTLA-4 (CD152), in various biological samples such as serum, plasma, cell culture supernatants, and tissue lysates. CTLA-4 is a critical immune checkpoint molecule that plays a fundamental role in regulating T-cell immune responses, making these kits invaluable for immunology and oncology research.

Content: CTLA ELISA kits employ the sandwich ELISA technique, which ensures high specificity and sensitivity for detecting CTLA-4 levels. The assay utilizes a dual-antibody system: the capture antibody pre-coated on the microplate binds to CTLA-4, and the detection antibody, conjugated to an enzyme (commonly horseradish peroxidase, HRP), binds to a different epitope on the target protein. 

The kits come complete with:

• Pre-coated ELISA plates
• Detection antibodies
• HRP conjugates
• TMB substrate solution
• Standard diluent and sample buffers
• Wash buffer and stop solution

Detailed protocols guide users through sample preparation, incubation times, and plate washing steps to maintain the precision and reliability of the assay.

Applications: CTLA ELISA kits are widely used in:

• Cancer Research: Given CTLA-4’s role as an immune checkpoint that downregulates immune responses, these kits help study the protein's involvement in tumor immune evasion mechanisms and in evaluating immunotherapy targets, particularly in therapies involving immune checkpoint inhibitors.
• Autoimmune Disease Studies: Elevated or altered CTLA-4 levels have implications in autoimmune diseases such as rheumatoid arthritis and lupus. ELISA assays provide insights into CTLA-4's regulatory functions in autoimmune pathologies.
• Immunological Research: The kits support investigations into the balance of T-cell activation and suppression, shedding light on T-cell tolerance and homeostasis in the immune system.
• Therapeutic Monitoring: In clinical research, CTLA-4 quantification is important for assessing the efficacy of immune checkpoint blockade therapies and for monitoring immune responses in patients undergoing immunotherapy.

By enabling accurate, reproducible quantification of CTLA-4, these ELISA kits provide researchers with essential data to advance our understanding of immune regulation and its impact on disease progression and treatment strategies.