EPCAM ELISA Kits

EPCAM (Epithelial Cell Adhesion Molecule) ELISA kits are diagnostic tools designed for the quantitative measurement of EPCAM in biological samples such as serum, plasma, cell lysates, and tissue homogenates. EPCAM is a transmembrane glycoprotein involved in cell adhesion and is highly expressed in epithelial tissues and certain carcinomas, making it a critical marker in oncology and regenerative medicine research.

Content: EPCAM ELISA kits utilize the sandwich ELISA method to provide high sensitivity and specificity for detecting EPCAM. These kits typically include a capture antibody that is pre-coated on the ELISA plate and a detection antibody conjugated to an enzyme, such as horseradish peroxidase (HRP), which facilitates the colorimetric detection.

Components of EPCAM ELISA kits commonly include:

• Pre-coated 96-well microplate with anti-EPCAM antibodies
• Standards and controls for generating a standard curve
• Biotin-conjugated detection antibody for specific binding
• Enzyme conjugate (e.g., Streptavidin-HRP)
• TMB substrate solution for color development
• Stop solution to terminate the reaction
• Wash and assay buffers for precise handling and reproducibility

Applications: EPCAM ELISA kits are utilized in several key areas of research:

• Cancer Research: EPCAM is overexpressed in many carcinomas, such as colon, breast, and ovarian cancers. These kits are used to measure EPCAM levels as a potential biomarker for cancer detection and prognosis.
• Stem Cell Research: EPCAM is a marker for epithelial and some stem cells, playing a role in studying stem cell properties and regenerative medicine.
• Cell Adhesion Studies: Understanding the role of EPCAM in cellular adhesion, migration, and communication within epithelial tissues.
• Immunotherapy Development: Evaluating EPCAM expression can aid in the development of targeted therapies, such as monoclonal antibodies or CAR-T cell therapies, against EPCAM-positive tumors.

EPCAM ELISA kits provide researchers with valuable quantitative data for understanding the expression and role of EPCAM in disease states, aiding in the identification of novel therapeutic targets and contributing to advancements in oncology and cell biology research.