EUREG ELISA Kits
EREG ELISA kits are designed to detect and quantify EREG concentrations in various biological samples, including serum, plasma, cell culture supernatants, and tissue homogenates. These kits typically employ a sandwich ELISA format, where specific antibodies capture EREG, and detection is facilitated through enzyme-linked secondary antibodies, resulting in a measurable signal proportional to the EREG concentration.
Key Components:
- Pre-coated Microplate: Wells pre-coated with anti-EREG antibodies to capture the target protein.
- Standards and Controls: Known concentrations of EREG to generate a standard curve and validate assay performance.
- Detection Antibodies: Enzyme-conjugated antibodies specific to EREG for signal generation.
- Substrate Solution: Reacts with the enzyme to produce a detectable signal, typically colorimetric.
- Buffers and Wash Solutions: Ensure optimal reaction conditions and remove unbound substances.
Applications:
- Cancer Research: Investigating the role of EREG in tumor growth, progression, and metastasis, as elevated EREG levels have been observed in various cancers.
- Immunology: Studying EREG's involvement in inflammatory responses and its potential as a therapeutic target.
- Developmental Biology: Exploring EREG's function in tissue development and repair processes.
Examples of EREG ELISA Kits:
- Mouse Epiregulin (EREG) ELISA Kit - Invitrogen: Quantifies mouse EREG in plasma, serum, and supernatant samples, with a sensitivity of 10 pg/mL.
- Mouse Epiregulin ELISA Kit (EREG) - Abcam: Measures mouse EREG in plasma and cell culture supernatants, with a sensitivity of less than 10 pg/mL.
- Human Epiregulin (EREG) ELISA Kit - Cusabio: Designed for the quantitative measurement of human EREG in serum, plasma, cell culture supernatants, and tissue homogenates, with a sensitivity of 3.9 pg/mL.
- Human Epiregulin ELISA Kit (EREG) - Abcam: A single-wash 90-minute ELISA designed for the quantitative measurement of human EREG in serum and cell culture supernatant, with a sensitivity of 5.8 pg/mL.
By providing reliable and quantitative measurements of EREG, ELISA kits are indispensable tools in advancing our understanding of its biological functions and facilitating research in various biomedical fields.
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