Fertility Markers ELISA Kits are specialized assays designed to measure biomarkers related to fertility in both men and women. These markers help in assessing reproductive health, diagnosing fertility issues, and guiding treatment decisions in fertility clinics and research settings.

Common Fertility Markers and Their ELISA Kits

• Luteinizing Hormone (LH):
  • Purpose: To measure LH levels, which play a crucial role in regulating the menstrual cycle and ovulation. Abnormal levels can indicate issues such as polycystic ovary syndrome (PCOS) or hypothalamic disorders.
• Follicle-Stimulating Hormone (FSH):
  • Purpose: To measure FSH levels, which are important for ovarian follicle development and spermatogenesis. Abnormal FSH levels can indicate ovarian reserve issues, primary ovarian insufficiency, or testicular dysfunction.
• Estradiol (E2):
  • Purpose: To measure estradiol levels, a key estrogen involved in regulating the menstrual cycle and supporting reproductive health. Abnormal estradiol levels can impact ovulation and fertility.
• Progesterone:
  • Purpose: To measure progesterone levels, crucial for maintaining the uterine lining and supporting early pregnancy. Low levels can indicate luteal phase defects or early pregnancy loss.
• Testosterone:
  • Purpose: To measure testosterone levels, which affect spermatogenesis and libido in men, and can also influence reproductive health in women. Abnormal levels can be associated with conditions like hypogonadism or PCOS.

Key Steps in the Fertility Marker ELISA Procedure

• Sample/Standard Addition: Standards and samples are added to wells pre-coated with capture antibody specific to the fertility marker.
• Incubation: The plate is incubated to allow the fertility marker to bind to the capture antibody.
• Washing: Wells are washed to remove unbound materials and reduce background noise.
• Detection Antibody Addition: A detection antibody specific to the fertility marker is added, binding to the captured marker.
• Washing: Excess detection antibody is removed by washing.
• Enzyme Conjugate Addition: Enzyme-conjugated secondary antibody is added.

Applications

• Fertility Assessment: Evaluating reproductive hormone levels to diagnose and manage fertility issues in both men and women.
• Ovarian Reserve Testing: Assessing ovarian reserve using markers like AMH to predict ovarian response to stimulation and fertility treatment outcomes.
• Monitoring Treatments: Tracking changes in hormone levels during fertility treatments to optimize outcomes and adjust protocols as needed.
• Research: Studying reproductive health, hormonal imbalances, and the effects of various interventions on fertility.

Important Considerations

• Sensitivity and Specificity: Ensure the ELISA kit is highly sensitive and specific for the fertility marker to provide accurate and reliable results.
• Sample Handling: Proper handling and storage of samples are crucial to prevent degradation of biomarkers and ensure accurate measurements.
• Assay Conditions: Follow the manufacturer's instructions carefully regarding incubation times, temperatures, and buffer compositions to ensure consistent and reliable results.