The FGL (Fibroblast Growth Factor-Like) ELISA Kit is a specialized assay designed for the quantitative measurement of FGL protein levels in various biological samples such as serum, plasma, cell culture supernatants, and tissue homogenates. FGL, also known as FGF-like protein, plays a significant role in cell proliferation, differentiation, and development, particularly in the context of nervous system function and repair, as well as in metabolic regulation.
Content of the Kit
- Pre-coated 96-Well Microplate:
- Wells coated with a specific monoclonal or polyclonal antibody against FGL, allowing for the capture of the target protein from the samples.
- Standards:
- Known concentrations of recombinant or purified FGL protein, used to generate a standard curve for quantifying FGL in unknown samples.
- Sample Diluent:
- A buffer solution used to dilute biological samples to optimal concentrations for accurate measurement.
- Detection Antibody:
- An antibody specific to a different epitope of the FGL protein, typically conjugated to biotin or directly to an enzyme like HRP.
- Streptavidin-HRP Conjugate (if using a biotinylated detection antibody):
- Binds to the biotinylated detection antibody, enabling detection through enzymatic color change.
- Substrate Solution (TMB):
- Tetramethylbenzidine, which reacts with HRP to produce a measurable color change.
Assay Procedure Summary
- Sample and Standard Addition: Add standards and samples to the wells. FGL in the samples binds to the antibody coated on the microplate.
- Wash: Unbound materials are removed by washing the wells.
- Detection Antibody Addition: Add the detection antibody specific to FGL, which binds to the captured protein.
- Wash: Unbound detection antibody is washed away.
- HRP-Streptavidin Conjugate Addition: (If using a biotinylated detection antibody) Add HRP-streptavidin conjugate, which binds to the biotinylated detection antibody.
- Wash: Remove unbound HRP-streptavidin conjugate by washing.
Performance Characteristics
- Sensitivity: Capable of detecting FGL at low picogram per milliliter (pg/mL) levels.
- Precision: The assay typically shows low intra-assay and inter-assay variability, with coefficients of variation (CV) below 10%.
- Linearity: Exhibits a linear response over the standard curve range, ensuring accurate quantification across different sample concentrations.
- Recovery: High recovery rates, typically above 80%, ensuring the assay's robustness across various sample types.
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