FXR ELISA Kits

Farnesoid X Receptor (FXR) is a nuclear receptor primarily expressed in the liver and intestine. FXR plays a critical role in regulating bile acid homeostasis, lipid metabolism, and glucose balance. Its activity impacts numerous physiological and pathological processes, including liver function, metabolic disorders, and inflammation. 

Content of FXR ELISA Kits

A typical FXR ELISA kit contains:

• 96-well microplate: Pre-coated with specific capture antibodies that bind FXR for high assay reliability.
• Standards: Recombinant FXR protein standards for establishing a calibration curve to ensure accurate quantification.
• Detection antibodies: Biotin-conjugated or enzyme-linked antibodies for specific detection of FXR bound to the capture antibody.
• Enzyme conjugate: Usually streptavidin-HRP (horseradish peroxidase) that binds to the biotinylated detection antibody, enabling a colorimetric reaction.
• TMB substrate: Used for the colorimetric detection that produces a measurable signal upon reaction with HRP.

Application of FXR ELISA Kits

FXR ELISA kits have diverse applications in biomedical and translational research, including:

• Liver disease studies: Quantifying FXR levels helps assess its role in hepatic diseases such as non-alcoholic fatty liver disease (NAFLD), cholestasis, and liver fibrosis. Researchers can monitor changes in FXR expression as a potential biomarker for disease progression and treatment response.
• Metabolic syndrome and diabetes: FXR’s regulation of lipid and glucose metabolism makes it a key marker in studying metabolic syndromes, obesity, and type 2 diabetes.
• Drug development: Evaluating FXR modulation by therapeutic compounds can aid in developing drugs targeting metabolic disorders, as FXR agonists or antagonists can influence metabolic and inflammatory pathways.
• Inflammation and immune response: Investigating FXR levels helps in understanding its immunomodulatory effects in conditions like inflammatory bowel disease (IBD) and other systemic inflammatory disorders.

The sensitivity and specificity of FXR ELISA kits make them essential for accurate quantification in research settings, ensuring reliable data for understanding FXR-related regulatory mechanisms in health and disease.