The GHRH (Growth Hormone-Releasing Hormone) ELISA Kit is used to measure the concentration of Growth Hormone-Releasing Hormone in biological samples. GHRH is a peptide hormone that stimulates the release of growth hormone (GH) from the anterior pituitary gland. It plays a crucial role in growth, metabolism, and various physiological processes.
Content of the Kit
- Pre-coated 96-Well Microplate:
- Wells pre-coated with an antibody specific to GHRH, allowing for the capture of GHRH from the samples.
- Standards:
- Solutions containing known concentrations of recombinant or purified GHRH, used to generate a standard curve for quantifying GHRH levels in samples.
- Sample Diluent:
- A buffer solution used to dilute biological samples to appropriate concentrations for accurate measurement.
- Detection Antibody:
- An antibody specific to a different epitope of GHRH, often conjugated to an enzyme such as HRP for detection.
- Enzyme Conjugate:
- If the detection antibody is biotinylated, a streptavidin-HRP conjugate may be included for signal detection.
Procedure
- Coating:
- Coat the wells of the microplate with an antibody specific to GHRH. Incubate to allow binding and then wash away unbound antibody.
- Blocking:
- Block any remaining uncoated surfaces in the wells with a blocking buffer to prevent non-specific binding.
- Addition of Standards and Samples:
- Add standards and samples to the wells. The GHRH in the samples will bind to the immobilized antibody.
- Incubation:
- Incubate the plate to allow GHRH to bind to the antibody.
- Addition of Detection Antibody:
- Add a detection antibody specific to GHRH, which binds to the captured GHRH.
Performance Characteristics
- Sensitivity: Capable of detecting low levels of GHRH, typically in the piogram per milliliter (pg/mL) range.
- Precision: Shows low intra-assay and inter-assay variability, with coefficients of variation (CV) generally below 10%.
- Linearity: Demonstrates linearity across the standard curve range, ensuring accurate quantification.
- Recovery: High recovery rates, usually above 85%, indicating reliable performance across different sample types.
Applications
- Growth Disorder Research: Evaluating GHRH levels in patients with growth hormone deficiencies or excess.
- Pituitary Function Studies: Investigating the role of GHRH in pituitary gland function and disorders.
- Metabolic Disease Research: Understanding the impact of GHRH on metabolism and related diseases.
- Pharmacological Studies: Assessing the effects of drugs on GHRH levels and growth hormone release.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|