GM-CSF ELISA Kits are designed for the quantitative measurement of Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) levels in various biological samples. GM-CSF is a cytokine that plays a crucial role in the differentiation, proliferation, and activation of granulocyte and macrophage lineages, impacting immune responses and hematopoiesis.
Technical Content
Key Features:
- High Sensitivity: Capable of detecting low levels of GM-CSF, ensuring accurate measurement even in samples with low cytokine concentrations.
- Specificity: High specificity for GM-CSF, minimizing cross-reactivity with other cytokines and proteins.
- Wide Range of Applications: Suitable for use with serum, plasma, cell culture supernatants, and other biological fluids.
Applications:
- Immunology Research: Used to study the role of GM-CSF in immune responses, inflammation, and autoimmune diseases.
- Clinical Research: Assists in investigating GM-CSF levels in various conditions, including cancer, infectious diseases, and inflammatory disorders.
- Drug Development: Facilitates the evaluation of therapeutic agents targeting GM-CSF pathways in preclinical and clinical studies.
Usage Protocol:
- Sample Preparation: Collect and prepare samples according to the kit instructions. Dilute if necessary.
- Assay Procedure: Add samples, standards, and controls to the wells of the ELISA plate pre-coated with GM-CSF-specific antibodies.
- Incubation: Incubate the plate to allow binding of GM-CSF to the antibodies.
- Detection: Add detection antibodies and enzyme substrate to generate a colorimetric signal proportional to the GM-CSF concentration.
- Analysis: Measure the absorbance using a microplate reader and calculate GM-CSF concentrations based on the standard curve.
Benefits:
- Accuracy: Provides reliable and reproducible results for the quantitative analysis of GM-CSF.
- Efficiency: Streamlined protocol with ready-to-use reagents reduces assay time and labor.
- Versatility: Compatible with a variety of sample types, making it suitable for diverse research needs.
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