GNMT ELISA Kits

GNMT (Glycine N-methyltransferase) ELISA kits are specialized immunoassay kits used for the quantitative detection of GNMT levels in various biological samples, including serum, plasma, cell lysates, and tissue homogenates. GNMT is an enzyme that plays a pivotal role in the regulation of S-adenosylmethionine (SAM) levels and in the detoxification of harmful compounds through methylation processes. It is primarily expressed in the liver and is implicated in liver function, metabolism, and hepatocellular carcinoma.

Content: GNMT ELISA kits are based on the sandwich ELISA technique, providing high specificity and sensitivity for detecting GNMT. The kits typically include:

• Pre-coated 96-well microplate with an anti-GNMT capture antibody.
• Standards and controls for creating a standard curve for quantification.
• Biotinylated detection antibody, designed to bind to a different epitope on the GNMT molecule.
• Streptavidin-HRP conjugate, for signal amplification.
• TMB substrate solution, which reacts with HRP to produce a measurable color change.
• Stop solution, for terminating the enzymatic reaction.
• Wash buffers and sample diluents to ensure optimal assay performance and reproducibility.

Applications: GNMT ELISA kits are utilized in various research areas, including:

• Liver Function and Disease Studies: GNMT’s significant role in liver metabolism makes these kits essential for understanding liver function and for studies related to liver diseases, including hepatocellular carcinoma and other liver disorders.
• Cancer Research: GNMT has been associated with tumor suppression and regulation of methyl group metabolism, making it a potential biomarker for cancer diagnosis and prognosis.
• Metabolic and Epigenetic Studies: Due to its involvement in regulating SAM levels, GNMT plays a critical role in methylation processes, impacting DNA methylation and gene expression.
• Pharmacological Research: The evaluation of GNMT expression can help in understanding the impact of drugs and therapies on liver health and metabolic processes.

Conclusion: GNMT ELISA kits provide a robust and precise method for the quantification of GNMT in biological samples. They are essential tools for research into liver metabolism, cancer, and the regulation of methylation processes. Their use in clinical and laboratory settings enhances our understanding of GNMT’s physiological and pathological roles, contributing to the development of diagnostic and therapeutic strategies.