GSTm ELISA Kits

GSTm (Glutathione S-Transferase Mu) ELISA Kits are advanced assay systems designed for the quantitative determination of GSTm proteins in various biological samples such as serum, plasma, tissue homogenates, and cell lysates. GSTm is a member of the glutathione S-transferase family, specifically the Mu class, which plays a vital role in the detoxification processes by catalyzing the conjugation of reduced glutathione to a wide range of electrophilic compounds. 

Content:

GSTm ELISA Kits use a sandwich enzyme-linked immunosorbent assay format, ensuring high specificity and sensitivity. The assay involves the following key steps:

• Capture Phase: The ELISA plate is pre-coated with high-affinity antibodies specific to GSTm. When a sample is added, the GSTm proteins bind to the immobilized antibodies.
• Detection Phase: A biotinylated detection antibody specific to another epitope on the GSTm protein is added, forming a sandwich complex.
• Signal Development: An enzyme-conjugated secondary antibody, typically linked to horseradish peroxidase (HRP), binds to the detection antibody. A substrate solution is then introduced, leading to a colorimetric reaction.

Application of GSTm ELISA Kits:

GSTm ELISA Kits are widely used in toxicology, oncology, and pharmacology research to study the role of GSTm in cellular detoxification and its involvement in various disease mechanisms. The primary applications include:

• Toxicological Studies: Monitoring GSTm levels helps in evaluating the cellular response to toxins and oxidative stress, providing insights into the detoxification processes within cells.
• Cancer Research: The overexpression of GSTm has been linked to drug resistance in certain cancers. Measuring GSTm levels can aid in understanding mechanisms of chemoresistance and contribute to the development of targeted therapeutic strategies.
• Pharmacokinetics and Drug Development: These kits are used to study the influence of pharmaceutical compounds on the expression of detoxification enzymes like GSTm, helping in assessing the safety and efficacy of new drugs.

The precision and reliability of GSTm ELISA Kits make them invaluable tools for researchers investigating the detoxification pathways and their impact on health and disease.