GSTp ELISA Kits

GSTp (Glutathione S-Transferase Pi) ELISA Kits are specialized assays used for the quantitative detection of GSTp proteins in various biological samples, including serum, plasma, cell lysates, and tissue homogenates. GSTp is a member of the glutathione S-transferase family, which plays a critical role in cellular detoxification by catalyzing the conjugation of glutathione to a wide range of harmful compounds.

Content:

GSTp ELISA Kits are designed with a high-sensitivity sandwich enzyme-linked immunosorbent assay format. The assay utilizes specific capture antibodies that bind to the GSTp protein, ensuring precise quantification. The target protein in the sample is captured by the pre-coated antibodies on the ELISA plate, followed by the addition of a detection antibody that binds to another epitope on the GSTp molecule. This is followed by the addition of an enzyme-linked secondary antibody, often conjugated with horseradish peroxidase (HRP). 

Application of GSTp ELISA Kits:

GSTp ELISA Kits are extensively used in research and clinical diagnostics for studying oxidative stress, cancer biology, and liver function. The following are key applications:

• Cancer Research: GSTp is often overexpressed in various cancers and is associated with drug resistance, particularly in chemotherapy. Measuring GSTp levels can help assess tumor progression and treatment response.
• Toxicology Studies: Due to its role in detoxification, GSTp levels can be used as a biomarker to monitor cellular response to exposure to toxic substances or oxidative stress.
• Pharmacokinetic and Drug Development Studies: GSTp ELISA Kits are utilized to evaluate the effect of drugs on the expression of detoxification enzymes, aiding in the assessment of drug safety and metabolism.
• Liver Function and Disease: As GSTp is involved in the metabolic processes of the liver, it serves as an important marker for liver function and can be used to investigate liver-related pathologies.

The high specificity and sensitivity of GSTp ELISA Kits make them essential tools for accurately quantifying GSTp, thereby facilitating in-depth studies on the enzyme’s role in detoxification pathways and its association with various diseases, including cancer and liver conditions.