GZMB ELISA Kits

GZMB (Granzyme B) ELISA kits are immunoassays designed for the quantitative measurement of Granzyme B in biological samples such as serum, plasma, cell culture supernatants, and tissue lysates. Granzyme B is a serine protease secreted by cytotoxic T lymphocytes and natural killer (NK) cells, playing a critical role in immune defense by inducing apoptosis in target cells. Measuring GZMB levels is vital in immunology research, cancer studies, and the evaluation of immune responses in infectious and autoimmune diseases.

Content of GZMB ELISA Kit: GZMB ELISA kits typically include:

• A 96-well microplate pre-coated with specific capture antibodies for Granzyme B.
• Recombinant Granzyme B standards for generating a standard curve.
• Detection antibodies conjugated to an enzyme, such as HRP (Horseradish Peroxidase), for signal amplification.
• Substrate solution (e.g., TMB – 3,3',5,5'-Tetramethylbenzidine) for colorimetric signal development.
• Stop solution to terminate the enzymatic reaction.
• Sample and assay diluents, wash buffers, and a detailed protocol for assay setup and sample handling.

Application of GZMB ELISA Kit: GZMB ELISA kits are used in various research and clinical applications, including:

• Immunology research: Studying the role of Granzyme B in cytotoxic immune responses and its involvement in immune-mediated cell death.
• Cancer research: Measuring GZMB levels to understand immune cell activity in tumor environments, contributing to the study of tumor immunity and potential immunotherapy targets.
• Infectious disease studies: Evaluating immune responses to viral and bacterial infections where cytotoxic cells play a role in controlling pathogens.
• Autoimmune disease research: Investigating GZMB's contribution to tissue damage and immune dysregulation in autoimmune conditions.
• Transplantation research: Assessing GZMB as a biomarker for transplant rejection, where heightened cytotoxic activity may indicate immune-mediated graft damage.
• Pharmacological studies: Monitoring the impact of drugs or therapies aimed at modulating cytotoxic cell activity, such as immune checkpoint inhibitors.

GZMB ELISA kits are invaluable tools for researchers studying immune mechanisms, cytotoxic cell function, and the role of Granzyme B in health and disease, providing essential data for understanding and developing targeted therapeutic approaches.