GZMK ELISA Kits

Granzyme K (GZMK) is a serine protease predominantly expressed in cytotoxic T cells and natural killer (NK) cells. GZMK plays a critical role in immune responses, particularly in mediating cell death and inflammation. GZMK ELISA (Enzyme-Linked Immunosorbent Assay) kits are specialized immunoassay tools designed to quantitatively measure the levels of Granzyme K in various biological samples such as serum, plasma, and cell culture supernatants. These kits are essential for monitoring immune activation and responses in both research and clinical applications.

Content of GZMK ELISA Kits

A typical GZMK ELISA kit includes:

• Pre-coated 96-well microplate: Immobilized with anti-GZMK capture antibodies for targeted antigen binding.
• Standard solutions: Recombinant GZMK protein for creating a calibration curve to ensure accurate quantification.
• Detection antibodies: Biotinylated secondary antibodies specific to GZMK for enhanced signal detection.
• Enzyme conjugate: Typically a streptavidin-horseradish peroxidase (HRP) for colorimetric reaction development.
• Substrate solution: TMB (3,3’,5,5’-Tetramethylbenzidine) for colorimetric signal generation upon HRP interaction.

Application of GZMK ELISA Kits

GZMK ELISA kits are utilized in various fields of research and diagnostics, particularly in the study of:

• Immuno-oncology: Assessing GZMK levels in patients to monitor tumor-infiltrating lymphocyte activity and effectiveness of immunotherapeutic interventions.
• Infectious diseases: Evaluating immune responses during viral and bacterial infections where GZMK expression is involved in pathogen clearance.
• Inflammatory conditions: Investigating the role of GZMK in chronic inflammation and autoimmune disorders where dysregulated cytotoxic T cell activity contributes to tissue damage.
• Translational research: Utilizing GZMK as a biomarker for immune activation in studies that bridge preclinical findings with clinical outcomes.

These kits offer high sensitivity and specificity, making them vital for quantitative analysis and contributing to a better understanding of immune cell functionality and pathophysiological mechanisms. The precise measurement of GZMK is crucial for designing targeted therapies, evaluating immune responses in various diseases, and advancing immunological research.