HMGB ELISA Kits

HMGB (High Mobility Group Box) ELISA kits are immunoassays designed for the quantitative measurement of HMGB proteins, such as HMGB1, in biological samples like serum, plasma, and cell lysates. HMGB proteins are non-histone chromatin-associated proteins involved in DNA organization, repair, and regulation of transcription. HMGB1, in particular, functions both as an intracellular DNA-binding protein and as an extracellular signaling molecule in inflammation and immune responses. These kits are essential for research into cell biology, inflammation, immune response, and cancer.

Content of HMGB ELISA Kits: HMGB ELISA kits typically include:

• A 96-well microplate pre-coated with specific capture antibodies targeting HMGB proteins.
• Recombinant HMGB protein standards for creating a standard curve.
• Detection antibodies conjugated to an enzyme (e.g., HRP – Horseradish Peroxidase) for signal detection.
• Substrate solution (e.g., TMB – 3,3',5,5'-Tetramethylbenzidine) for colorimetric signal development.
• Stop solution to halt the enzymatic reaction.
• Sample and assay diluents, wash buffers, and detailed protocols for sample preparation and assay procedures.

Application of HMGB ELISA Kits: HMGB ELISA kits are used in various research and clinical applications, including:

• Inflammatory disease research: Measuring extracellular HMGB1 levels to study its role as a pro-inflammatory mediator and its involvement in conditions such as sepsis, rheumatoid arthritis, and systemic lupus erythematosus.
• Cancer research: Investigating HMGB proteins’ roles in tumor progression, metastasis, and immune evasion, as HMGB1 can act as a damage-associated molecular pattern (DAMP) to promote inflammation and tumor growth.
• Cell biology: Studying the intracellular functions of HMGB proteins in chromatin remodeling, transcriptional regulation, and DNA repair.
• Neuroscience: Analyzing HMGB1’s involvement in neuroinflammatory and neurodegenerative processes, including diseases like Alzheimer’s and Parkinson’s.
• Immunology: Understanding HMGB1’s function in immune response modulation, particularly its release from necrotic cells and interaction with receptors like RAGE and TLRs.
• Pharmacological research: Evaluating potential therapeutics that target HMGB1 signaling pathways for inflammatory and autoimmune diseases.

HMGB ELISA kits are powerful tools for researchers studying inflammation, cell death, immune modulation, and the roles of HMGB proteins in health and disease. They provide valuable data that can inform the development of new diagnostics and therapies.