HRP ELISA Kits
HRP (Horseradish Peroxidase) ELISA kits are immunoassays designed to detect and measure the presence of specific analytes using HRP as an enzyme conjugate for signal amplification. HRP is a commonly used enzyme in ELISA assays due to its ability to catalyze the conversion of colorimetric or chemiluminescent substrates, enabling sensitive and precise detection. These kits are pivotal in various research and diagnostic applications, from quantifying proteins, hormones, and cytokines to detecting antibodies in samples such as serum, plasma, cell culture supernatants, and tissue extracts.
Content of HRP ELISA Kits
- Pre-coated Microplate: A 96-well plate pre-coated with a capture antibody specific to the target analyte.
- Detection Antibody: An analyte-specific biotinylated antibody that binds to the target and works in conjunction with the HRP conjugate.
- HRP Conjugate: An enzyme-linked streptavidin or secondary antibody conjugated to horseradish peroxidase for signal development.
- Substrate Solution: TMB (3,3’,5,5’-tetramethylbenzidine) substrate, which is converted to a colored product in the presence of HRP.
- Stop Solution: An acidic solution to stop the enzymatic reaction, stabilizing the color for absorbance reading.
Applications of HRP ELISA Kits
- Protein Quantification: These kits are extensively used to quantify proteins such as cytokines, growth factors, and other bioactive molecules in biological samples.
- Antibody Detection: HRP-based ELISA kits are valuable for detecting and measuring antibodies in immunological studies, aiding in vaccine development and infectious disease research.
- Hormone Assays: Used for the sensitive detection of hormones and biomarkers involved in metabolic, reproductive, and endocrine studies.
- Clinical Diagnostics: HRP ELISA kits play a critical role in clinical diagnostics by measuring markers associated with various diseases, including infections, autoimmune disorders, and cancers.
- Pharmaceutical Research: Used in drug development and screening to evaluate the efficacy of therapeutic compounds by monitoring the concentration of specific proteins or antibodies.
HRP ELISA kits are widely used due to their reliability, sensitivity, and broad application potential, making them essential tools in research and diagnostic laboratories.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|