IARS ELISA Kits

IARS ELISA kits employ the sandwich enzyme-linked immunosorbent assay (ELISA) technique. In this method, microtiter plates are pre-coated with antibodies specific to IARS. When samples or standards are introduced, IARS present in the sample binds to these capture antibodies. Subsequently, a biotin-conjugated detection antibody specific to IARS is added, forming a sandwich complex. This complex is then bound by an avidin-horseradish peroxidase (HRP) conjugate. Upon addition of a substrate solution, a colorimetric reaction occurs, with the intensity of the color being directly proportional to the IARS concentration in the sample.

Kit Components:

• Pre-coated 96-well microtiter plate: Immobilized with anti-IARS capture antibodies.
• IARS standards: For generating a standard curve to determine sample concentrations.
• Biotin-conjugated anti-IARS detection antibody: Binds to a different epitope on the IARS molecule.
• Avidin-HRP conjugate: Facilitates the enzymatic reaction for detection.
• Substrate solution: Typically tetramethylbenzidine (TMB), which reacts with HRP to produce a measurable color change.
• Stop solution: Usually an acidic solution to halt the enzymatic reaction.
• Wash buffers and diluents: For sample and reagent preparation.

Applications:

IARS ELISA kits are utilized in various research and clinical settings, including:

• Molecular Biology: Investigating the role of IARS in protein synthesis and its regulation.
• Disease Research: Studying conditions where IARS expression is altered, such as certain cancers or metabolic disorders.
• Pharmacology: Evaluating the impact of drugs on IARS activity or expression levels.
• Biotechnology: Monitoring IARS levels in genetically modified organisms or cell lines.

These kits offer high sensitivity and specificity, enabling accurate quantification of IARS in complex biological matrices. For instance, the Human IARS ELISA Kit from Assay Genie is designed for high sensitivity and simple protocol, suitable for measuring IARS in serum, plasma, and cell lysate samples