IL10RB ELISA Kits

IL10RB (Interleukin-10 Receptor Subunit Beta) ELISA kits are immunoassays designed for the quantitative detection of IL10RB in biological samples such as serum, plasma, and cell culture supernatants. IL10RB is a part of the receptor complex for interleukin-10 (IL-10), a key anti-inflammatory cytokine involved in immune regulation. IL10RB pairs with the alpha subunit (IL10RA) to form a functional receptor complex, mediating the signaling of IL-10 and contributing to the suppression of inflammatory responses. 

Content of IL10RB ELISA Kits: IL10RB ELISA kits typically include:

• A 96-well microplate pre-coated with capture antibodies specific to IL10RB.
• Recombinant IL10RB standards for generating a standard curve.
• Detection antibodies, often conjugated with an enzyme such as HRP (Horseradish Peroxidase) for signal development.
• Substrate solution (e.g., TMB – 3,3',5,5'-Tetramethylbenzidine) for colorimetric detection.
• Stop solution to terminate the enzymatic reaction.
• Sample diluents, wash buffers, assay buffers, and detailed instructions for assay preparation and sample handling.

Application of IL10RB ELISA Kits: IL10RB ELISA kits are used in various research and clinical applications, including:

• Immunology research: Studying the role of IL10RB in mediating IL-10 signaling and its contribution to the regulation of immune responses, particularly its role in anti-inflammatory processes.
• Autoimmune disease studies: Investigating IL10RB expression in autoimmune conditions such as rheumatoid arthritis, lupus, and inflammatory bowel disease, where the IL-10 pathway may be disrupted.
• Infectious disease research: Exploring IL10RB’s role in responses to viral and bacterial infections, as its signaling can impact the control and resolution of inflammation.
• Cancer research: Analyzing IL10RB expression in tumor microenvironments to understand its role in immune evasion and tumor progression, as some cancers may exploit IL-10 signaling for immune suppression.

IL10RB ELISA kits provide valuable tools for researchers studying the IL-10 signaling pathway and its implications in disease and health, contributing to advancements in understanding immune regulation and potential therapeutic strategies.