IL10RB (Interleukin-10 Receptor Beta) ELISA kits are used to measure the levels of IL10RB in biological samples. IL10RB is a component of the interleukin-10 receptor complex, which plays a crucial role in modulating immune responses and inflammation. Accurate measurement of IL10RB is important for studying immune system function and various diseases associated with immune regulation, such as autoimmune disorders and cancer.
Components
- Coated Microplate:
- A 96-well plate pre-coated with antibodies specific to IL10RB. This allows IL10RB in the sample to be captured on the plate.
- Standards:
- Recombinant or purified IL10RB proteins with known concentrations used to generate a standard curve for quantifying IL10RB levels in samples.
- Detection Antibody:
- A secondary antibody specific to IL10RB, typically conjugated to an enzyme such as horseradish peroxidase (HRP) or alkaline phosphatase (AP) for detection.
- Substrate Solution:
- Contains a substrate that reacts with the enzyme linked to the detection antibody, producing a color change proportional to the amount of IL10RB.
- Stop Solution:
- An acid solution that stops the enzyme reaction and stabilizes the color for measurement.
- Wash Buffer:
- Used to wash away unbound substances and reduce background noise, ensuring that only specifically bound IL10RB is measured.
.Technical Considerations
- Sensitivity:
- The minimum detectable concentration of IL10RB by the assay.
- Specificity:
- The ability of the kit to specifically detect IL10RB without cross-reacting with other proteins.
- Reproducibility:
- Consistency of results across different assays and sample conditions.
- Interferences:
- Potential effects of other substances in the sample that might affect assay accuracy.
Applications
- Research: Investigating the role of IL10RB in immune regulation, inflammation, and associated diseases.
- Clinical Diagnostics: Assessing IL10RB levels in conditions related to immune system dysfunction, including autoimmune diseases and cancer.
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