IL2RA ELISA Kits

IL2RA, also known as CD25, is the alpha chain of the IL-2 receptor, a critical component in T cell activation and immune regulation. IL2RA ELISA Kits are designed for the quantitative measurement of soluble IL-2 receptor alpha (sIL2RA) levels in biological samples such as serum, plasma, and cell culture supernatants. As a key biomarker of immune activity, IL2RA is often studied in relation to immune disorders, including autoimmune diseases, cancer, and transplantation rejection.

Content of IL2RA ELISA Kits

IL2RA ELISA Kits typically contain:

• Pre-coated ELISA Plate: Wells are coated with an anti-IL2RA capture antibody that ensures high specificity for detecting IL2RA in complex biological samples.
• Standards and Controls: Serial dilutions of IL2RA standard protein for generating a standard curve, essential for accurate quantification across sample concentrations.
• Detection Antibody: Often a biotinylated or enzyme-linked secondary antibody specific to IL2RA, which binds to the captured IL2RA and allows for signal detection.
• Substrate Solution: Colorimetric substrates such as TMB (3,3',5,5'-Tetramethylbenzidine) are used to produce a detectable color change proportional to the IL2RA concentration.
• Stop Solution and Wash Buffers: These components help terminate the reaction and remove unbound substances, reducing background noise and increasing assay sensitivity.

Application of IL2RA ELISA Kits

IL2RA ELISA Kits are widely used in research fields such as:

• Autoimmune Disease Research: Elevated levels of IL2RA are associated with autoimmune disorders like rheumatoid arthritis, systemic lupus erythematosus (SLE), and multiple sclerosis. Quantifying IL2RA aids in understanding disease activity, immune regulation, and pathogenesis.
• Cancer Immunology: In cancers, particularly lymphomas and leukemias, IL2RA is overexpressed on activated T cells and can serve as a marker for immune response and disease progression. IL2RA measurement is also valuable in assessing the immune landscape and monitoring immune-based therapies.
• Transplantation and Immune Monitoring: IL2RA levels can act as biomarkers for graft rejection and immune activation in transplant patients, offering insight into immune status and transplant tolerance. It can be critical in the early detection of rejection and assessing the efficacy of immunosuppressive therapy.

Overall, IL2RA ELISA Kits provide precise and reproducible measurements, supporting research in immune system modulation, disease diagnostics, and therapy monitoring.