JAK ELISA Kits

JAK (Janus Kinase) ELISA kits are immunoassays developed for the quantitative detection of JAK family proteins, including JAK1, JAK2, JAK3, and TYK2, in various biological samples such as serum, plasma, cell lysates, and tissue homogenates. The JAK proteins are tyrosine kinases that play a fundamental role in the JAK-STAT signaling pathway, which is critical for the regulation of immune response, hematopoiesis, and cellular growth. Abnormalities in JAK signaling are implicated in various diseases, including autoimmune disorders, cancers, and inflammatory conditions.

Content of JAK ELISA Kits

• Pre-coated Microplate: A 96-well plate pre-coated with a capture antibody specific to the targeted JAK protein.
• Detection Antibody: A biotinylated secondary antibody that binds to the specific JAK protein, facilitating signal detection.
• Enzyme Conjugate: Streptavidin conjugated to horseradish peroxidase (HRP) to amplify the detection signal.
• Standards: Calibrated JAK protein standards for creating a standard curve to quantify sample concentrations.
• Substrate Solution: TMB (3,3’,5,5’-tetramethylbenzidine) for color development.

Applications of JAK ELISA Kits

• Cancer Research: JAK signaling dysregulation is linked to various cancers, including leukemia and lymphoma. JAK ELISA kits help in studying JAK protein expression levels and activity, aiding in understanding tumor progression and drug response.
• Autoimmune Disease Studies: The role of JAKs in autoimmune conditions such as rheumatoid arthritis and psoriasis is significant. ELISA kits facilitate the measurement of JAK expression or activation to evaluate disease mechanisms and therapeutic interventions.
• Pharmaceutical Development: In drug discovery, JAK inhibitors are key targets for new therapies. JAK ELISA kits are used to measure the efficacy of potential inhibitors by quantifying JAK protein levels in preclinical and clinical studies.
• Signal Transduction Research: JAK ELISA kits are essential for dissecting the JAK-STAT pathway and its involvement in various cellular processes, including proliferation and immune regulation.
• Hematopoiesis and Immune Function Studies: Since JAK proteins play a crucial role in the formation of blood cells and immune cell regulation, their quantification is important in studies related to bone marrow disorders and immune system functions.

JAK ELISA kits are vital for researchers studying signaling pathways, immune function, and disease progression, offering reliable and reproducible data to support advances in biomedical research and therapeutic development.