The KCNN (Potassium Calcium-Activated Channel, Subfamily N, Member 1) ELISA Kit is designed for the quantitative measurement of KCNN1 (also known as SK1 or Small Conductance Calcium-Activated Potassium Channel 1) in biological samples. KCNN1 is a type of potassium channel that is activated by intracellular calcium and plays a role in various physiological processes including smooth muscle contraction, neurotransmitter release, and cellular signaling.

Content of the Kit

• Pre-coated 96-Well Microplate:
  • Wells pre-coated with an antibody specific to KCNN1, allowing for capture of KCNN1 from the samples.
• Standards:
  • Solutions containing known concentrations of recombinant or purified KCNN1, used to generate a standard curve for quantifying KCNN1 levels in samples.
• Sample Diluent:
  • A buffer solution used to dilute biological samples to appropriate concentrations for accurate measurement.
• Detection Antibody:
  • An antibody specific to a different epitope of KCNN1, often conjugated to an enzyme such as HRP for detection.
• Enzyme Conjugate:
  • If the detection antibody is biotinylated, a streptavidin-HRP conjugate may be included for signal detection.

Procedure

• Coating:
  • Coat the wells of the microplate with an antibody specific to KCNN1. Incubate to allow binding and then wash away unbound antibody.
• Blocking:
  • Block any remaining uncoated surfaces in the wells with a blocking buffer to prevent non-specific binding.
• Addition of Standards and Samples:
  • Add standards and samples to the wells. The KCNN1 in the samples will bind to the immobilized antibody.
• Incubation:
  • Incubate the plate to allow KCNN1 to bind to the antibody.
• Addition of Detection Antibody:
  • Add a detection antibody specific to KCNN1, which binds to the captured KCNN1.

Performance Characteristics

• Sensitivity: Capable of detecting low levels of KCNN1, typically in the picogram per milliliter (pg/mL) range.
• Precision: Shows low intra-assay and inter-assay variability, with coefficients of variation (CV) generally below 10%.
• Linearity: Demonstrates linearity across the standard curve range, ensuring accurate quantification.
• Recovery: High recovery rates, usually above 85%, indicating reliable performance across different sample types.

Applications

• Ion Channel Research: Studying the role of KCNN1 in ion channel function and cellular signaling.
• Neurological Studies: Investigating the involvement of KCNN1 in neuronal excitability and neurotransmitter release.
• Cardiovascular Research: Assessing the role of KCNN1 in smooth muscle function and cardiovascular health.
• Drug Development: Evaluating the impact of potential therapeutics on KCNN1 expression and function.