KCNN ELISA Kits

The KCNN ELISA Kit is a specialized immunoassay designed for the quantitative detection of KCNN proteins, specifically the small-conductance calcium-activated potassium channels (SK channels) such as KCNN1, KCNN2, and KCNN3. These channels are integral membrane proteins that play a crucial role in regulating neuronal excitability and various physiological processes by mediating calcium-activated potassium currents.

Kit Components:

• Pre-coated 96-well microplate: Wells are pre-coated with specific antibodies against the target KCNN protein.
• Standards and Controls: Known concentrations of the target protein to generate a standard curve for quantification.
• Detection Reagents: Biotinylated detection antibodies and streptavidin-HRP conjugate for signal amplification.
• Substrate Solution: Typically TMB (3,3',5,5'-Tetramethylbenzidine) for color development.
• Stop Solution: To terminate the enzymatic reaction, allowing for absorbance measurement.
• Wash Buffer and Diluent: For washing steps and sample/standard dilution.

Applications:

• Neuroscience Research: Quantifying KCNN channel expression in neuronal tissues to study their role in synaptic plasticity and neuronal signaling.
• Cardiovascular Studies: Investigating the involvement of KCNN channels in cardiac function and their potential link to arrhythmias.
• Pharmacological Testing: Evaluating the effects of drugs targeting KCNN channels, which are potential therapeutic targets for various disorders.
• Disease Biomarker Identification: Assessing KCNN protein levels in pathological conditions to identify potential biomarkers.

These kits are essential tools for researchers aiming to elucidate the physiological and pathological roles of KCNN channels in various tissues and disease states.