Kim ELISA Kits
The KIM-1 ELISA Kit is a specialized immunoassay for the quantitative measurement of Kidney Injury Molecule-1 (KIM-1) levels in biological samples. KIM-1, also known as HAVCR1 (Hepatitis A Virus Cellular Receptor 1), is a type 1 transmembrane protein highly upregulated in proximal tubular epithelial cells in response to kidney injury. It serves as an early biomarker for acute kidney injury (AKI) and chronic kidney disease (CKD), as its levels rise rapidly in response to renal injury, often before traditional markers such as creatinine.
Content of KIM-1 ELISA Kit
A standard KIM-1 ELISA Kit includes:
- Standards and Controls: Calibrated standards to generate a reliable standard curve, along with positive and negative controls to ensure assay accuracy and reproducibility.
- Substrate Solution: Chromogenic or chemiluminescent substrate that reacts with the enzyme to produce a colorimetric signal proportional to KIM-1 concentration.
- Stop Solution: Terminates the enzymatic reaction, allowing for consistent and accurate measurement.
- Wash Buffer and Sample Diluent: Solutions optimized to minimize background noise and ensure specificity.
These components are formulated to provide reliable, sensitive, and reproducible quantification of KIM-1 in various sample types, including serum, plasma, urine, and tissue lysates.
Application of KIM-1 ELISA Kit
The KIM-1 ELISA Kit is widely used in nephrology and pharmaceutical research, with applications in:
- Acute Kidney Injury (AKI) Diagnosis and Monitoring: KIM-1 is one of the earliest and most sensitive biomarkers for AKI. KIM-1 ELISA Kits help researchers and clinicians detect AKI in its early stages, supporting early intervention and monitoring of kidney function.
- Chronic Kidney Disease (CKD) Research: Elevated KIM-1 levels correlate with CKD progression, allowing researchers to study the role of KIM-1 in kidney function decline and evaluate the effectiveness of therapeutic interventions.
- Drug Toxicity Testing: KIM-1 is commonly used as a biomarker to assess nephrotoxicity in drug development. The KIM-1 ELISA Kit allows researchers to monitor kidney injury responses to drug compounds, supporting the development of safer pharmaceuticals.
The KIM-1 ELISA Kit is a critical tool in kidney disease research, diagnostics, and toxicology, offering sensitive and accurate quantification of KIM-1 levels to advance understanding in renal health and therapeutic development.
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