Overview of MCF ELISA Kits
MCF ELISA Kits are designed for the quantitative measurement of Macrophage Chemotactic Factor (MCF) levels in various biological samples. MCF is a chemokine that plays a pivotal role in the recruitment and activation of macrophages, influencing immune responses and inflammation.
Technical Content
Key Features:
- High Sensitivity: Capable of detecting low levels of MCF, ensuring precise quantification even in samples with low chemokine concentrations.
- Specificity: High specificity for MCF, minimizing cross-reactivity with other chemokines and proteins.
- Wide Range of Applications: Suitable for use with serum, plasma, cell culture supernatants, and other biological fluids.
Applications:
- Immunology Research: Used to study the role of MCF in immune responses, particularly in macrophage recruitment and activation.
- Inflammation Studies: Facilitates the investigation of MCF involvement in inflammatory diseases and conditions.
- Cancer Research: Assists in understanding the role of MCF in tumor-associated macrophage infiltration and tumor progression.
Usage Protocol:
- Sample Preparation: Collect and prepare samples according to the kit instructions. Dilute if necessary.
- Assay Procedure: Add samples, standards, and controls to the wells of the ELISA plate pre-coated with MCF-specific antibodies.
- Incubation: Incubate the plate to allow binding of MCF to the antibodies.
- Detection: Add detection antibodies and enzyme substrate to generate a colorimetric signal proportional to the MCF concentration.
- Analysis: Measure the absorbance using a microplate reader and calculate MCF concentrations based on the standard curve.
Benefits:
- Precision: Provides reliable and reproducible results for the accurate quantification of MCF.
- Efficiency: Simplified protocol with ready-to-use reagents reduces assay time and labor.
- Versatility: Compatible with a variety of sample types, making it suitable for diverse research applications.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|