The MGP (Matrix Gla Protein) ELISA Kit is designed for the quantitative measurement of MGP levels in various biological samples such as serum, plasma, and tissue homogenates. MGP is a vitamin K-dependent protein involved in inhibiting vascular calcification and regulating bone formation. It plays a crucial role in maintaining vascular health and is of particular interest in studies related to cardiovascular diseases, bone metabolism, and kidney function.
Content of the Kit
- Pre-coated 96-Well Microplate:
- Wells are pre-coated with a monoclonal or polyclonal antibody specific to MGP, allowing the capture of the target protein from the samples.
- Standards:
- Pre-diluted solutions of recombinant or purified MGP, used to create a standard curve for the quantification of MGP in unknown samples.
- Sample Diluent:
- A buffer solution used to dilute samples to appropriate concentrations for accurate measurement.
- Detection Antibody:
- An antibody specific to a different epitope of MGP, often conjugated to biotin or directly to an enzyme like HRP, enabling detection.
- Streptavidin-HRP Conjugate (if using a biotinylated detection antibody):
- This conjugate binds to the biotin on the detection antibody, enabling enzymatic color development.
- Substrate Solution (TMB):
- Tetramethylbenzidine, which reacts with HRP to produce a color change that can be measured.
Assay Procedure Summary
- Sample and Standard Addition: Add standards and samples to the wells. The MGP in the samples binds to the antibody coated on the microplate.
- Wash: Wash the wells to remove unbound components.
- Detection Antibody Addition: Add the detection antibody specific to MGP, which binds to the captured protein.
- Wash: Wash the wells again to remove any unbound detection antibody.
- HRP-Streptavidin Conjugate Addition: (If a biotinylated detection antibody is used) Add HRP-streptavidin conjugate, which binds to the biotinylated detection antibody.
- Wash: Remove unbound HRP-streptavidin conjugate by washing.
Performance Characteristics
- Sensitivity: Capable of detecting MGP levels in the low picogram per milliliter (pg/mL) range.
- Precision: Intra-assay and inter-assay variability are generally low, with coefficients of variation (CV) typically below 10%.
- Linearity: The assay is linear over the standard curve range, allowing for accurate quantification across different sample concentrations.
- Recovery: High recovery rates, often >85%, indicating accurate measurement across various sample types.
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