MPG ELISA Kits

The MPG ELISA Kit is a specialized immunoassay designed for the quantitative measurement of N-methylpurine DNA glycosylase (MPG) levels in biological samples. MPG, also known as 3-methyladenine DNA glycosylase, is a DNA repair enzyme that plays a crucial role in the base excision repair (BER) pathway by recognizing and excising damaged purine bases, such as 3-methyladenine, from DNA. 

Content of MPG ELISA Kit

A typical MPG ELISA Kit includes:

• Standards and Controls: Calibrated standards to generate a reliable standard curve, along with positive and negative controls to ensure assay precision and reproducibility.
• Substrate Solution: Chromogenic or chemiluminescent substrate that reacts with the enzyme to produce a detectable signal proportional to MPG concentration.
• Stop Solution: Stops the enzymatic reaction for consistent and accurate measurement.
• Wash Buffer and Sample Diluent: Solutions to optimize assay conditions, minimizing nonspecific binding and enhancing specificity.

These components are designed to provide reliable, reproducible quantification of MPG in various sample types, including cell lysates, tissue homogenates, and serum.

Application of MPG ELISA Kit

MPG ELISA Kits have diverse applications in research fields related to DNA repair, cancer, and cellular stress responses:

• DNA Damage and Repair Studies: MPG plays a critical role in the base excision repair pathway, which repairs damaged bases to prevent mutations. Quantifying MPG with ELISA kits helps researchers study DNA repair mechanisms and the cellular response to genotoxic stress.
• Cancer Research: Alterations in DNA repair enzymes like MPG are associated with carcinogenesis. MPG ELISA Kits allow researchers to investigate the role of MPG in cancer development, progression, and treatment resistance, as impaired repair pathways can lead to genetic instability in cancer cells.
• Aging and Cellular Senescence: DNA damage accumulation and repair capacity are linked to aging and senescence. MPG ELISA kits are useful for studies exploring the connection between DNA repair enzyme levels, such as MPG, and age-related cellular changes.

The MPG ELISA Kit is a valuable tool for researchers studying DNA repair, genomic stability, and cellular responses to DNA damage, offering precise and sensitive quantification to support advancements in oncology, aging, and genetic research.