The MSP (Mucin-1 Specific Peptide) ELISA Kit is designed for the quantitative measurement of MSP levels in various biological samples, such as serum, plasma, and cell culture supernatants. MSP refers to specific peptides or fragments derived from mucin-1 (MUC1), a highly glycosylated cell surface protein involved in various physiological processes and associated with several diseases, including cancer.
Content of the Kit
- Pre-coated 96-Well Microplate:
- Wells pre-coated with an antibody specific to MSP, allowing capture of the peptide from the sample.
- Standards:
- Solutions containing known concentrations of recombinant or synthetic MSP, used to generate a standard curve for quantifying MSP in samples.
- Sample Diluent:
- A buffer solution used to dilute biological samples to appropriate concentrations for accurate measurement.
- Detection Antibody:
- An antibody specific to a different epitope of MSP, often conjugated to an enzyme such as HRP for detection.
- Enzyme Conjugate:
- If the detection antibody is biotinylated, a streptavidin-HRP conjugate is used for detection.
- Substrate Solution (TMB):
- Tetramethylbenzidine, which reacts with HRP to produce a colorimetric signal proportional to the amount of MSP.
Assay Procedure Summary
- Sample and Standard Addition: Add standards and samples to the pre-coated wells. MSP in the samples is captured by the immobilized antibody.
- Wash: Remove unbound substances by washing the wells.
- Detection Antibody Addition: Add a detection antibody specific to MSP, which binds to the captured peptide.
- Wash: Wash away excess detection antibody.
- Enzyme Conjugate Addition: If using a biotinylated detection antibody, add streptavidin-HRP to bind to the biotinylated antibody.
- Wash: Remove unbound enzyme conjugate by washing.
Performance Characteristics
- Sensitivity: Can detect low levels of MSP, typically in the picogram per milliliter (pg/mL) range.
- Precision: Low intra-assay and inter-assay variability, with coefficients of variation (CV) generally below 10%.
- Linearity: The assay is linear across the standard curve range, ensuring accurate quantification.
- Recovery: High recovery rates, usually above 80%, ensuring the assay's reliability across various sample types.
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