MYCN ELISA Kits

MYCN ELISA kits are specialized immunoassays designed for the quantitative measurement of MYCN (N-Myc proto-oncogene protein) levels in biological samples such as cell lysates and tissue extracts. MYCN is a member of the MYC family of transcription factors, playing a crucial role in the regulation of cell growth, proliferation, and apoptosis. It is particularly important in cancer research due to its significant overexpression and gene amplification in specific cancers, notably neuroblastoma, where it serves as a key prognostic marker.

Content of MYCN ELISA Kits: MYCN ELISA kits typically include:

• A 96-well microplate pre-coated with capture antibodies specific to MYCN.
• Recombinant MYCN protein standards for creating a standard curve.
• Detection antibodies conjugated with an enzyme, such as HRP (Horseradish Peroxidase), for colorimetric signal development.
• Substrate solution (e.g., TMB – 3,3',5,5'-Tetramethylbenzidine) for visual signal detection.
• Stop solution to terminate the enzymatic reaction.
• Sample and assay diluents, wash buffers, and comprehensive instructions for assay setup and sample processing.

Application of MYCN ELISA Kits: MYCN ELISA kits are used in a variety of research and clinical applications, including:

• Cancer research: Quantifying MYCN expression in tumor samples to investigate its role in oncogenesis, tumor progression, and metastasis, particularly in neuroblastoma and other MYCN-amplified malignancies.
• Prognostic biomarker studies: Evaluating MYCN levels as a predictive biomarker for cancer outcomes, since high MYCN expression is often associated with aggressive disease and poorer prognosis.
• Drug discovery and development: Screening potential therapeutic agents that target MYCN or its downstream signaling pathways to assess their effects on MYCN expression.
• Cell cycle and growth regulation studies: Understanding the role of MYCN in cellular proliferation, differentiation, and survival.
• Neurodevelopmental research: Investigating MYCN’s involvement in neural development and its regulation of cell cycle processes in neuronal cells.

MYCN ELISA kits are invaluable tools for researchers focusing on cancer biology, cell growth regulation, and translational studies, offering insights into disease mechanisms and aiding in the development of targeted therapies.