NADH ELISA Kits
NADH (Nicotinamide Adenine Dinucleotide, reduced form) is a vital coenzyme in cellular metabolism, playing an essential role in energy production through the electron transport chain and in redox reactions. NADH serves as an important indicator of metabolic activity, mitochondrial function, and cellular health.
Content of NADH ELISA Kits
A typical NADH ELISA kit includes:
- Detection antibodies: Biotin-conjugated antibodies specific for NADH, enabling detection.
- Enzyme conjugate: Streptavidin-HRP (horseradish peroxidase) to bind to biotin-labeled detection antibodies for colorimetric signal generation.
- Substrate solution: TMB (3,3’,5,5’-Tetramethylbenzidine) used for visual signal generation upon reaction with HRP.
- Stop solution: Acidic solution (e.g., sulfuric acid) to terminate the enzyme reaction and stabilize the color for reading.
- Buffers: Assay and wash buffers for sample preparation, dilution, and thorough washing to reduce background noise.
Application of NADH ELISA Kits
NADH ELISA kits have a range of applications in scientific and clinical research:
- Mitochondrial function: NADH levels reflect mitochondrial health and oxidative phosphorylation efficacy. Analyzing NADH is crucial for research on mitochondrial disorders, aging, and neurodegenerative diseases.
- Oxidative stress studies: Changes in NADH levels can indicate redox imbalances and oxidative stress, making the ELISA kits valuable for exploring the mechanisms behind oxidative damage in cells.
- Cancer research: Altered NADH metabolism is linked to cancer cell proliferation and tumor progression. Quantifying NADH can contribute to understanding cancer metabolism and potential therapeutic targets.
- Drug development: NADH ELISA kits can be employed in preclinical studies to monitor the effects of drugs targeting metabolic pathways or mitochondrial function.
The high sensitivity and specificity of NADH ELISA kits make them indispensable for accurately measuring NADH concentrations, supporting reliable data for metabolic and mitochondrial studies.
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