NEIL ELISA Kits

NEIL ELISA Kits are specialized immunoassays developed to detect and quantify NEIL (Nei-like) DNA glycosylase enzymes, specifically NEIL1, NEIL2, and NEIL3, which are part of the base excision repair (BER) pathway. These enzymes are crucial in repairing oxidative DNA damage by recognizing and removing oxidized DNA bases, maintaining genomic stability, and preventing mutations. NEIL proteins are implicated in various physiological and pathological processes, including cancer, aging, and neurodegeneration, where oxidative stress and DNA repair efficiency are critical.

Content:

NEIL ELISA Kits generally include highly specific antibodies that target NEIL proteins, ensuring precise and sensitive quantification of NEIL1, NEIL2, or NEIL3 in biological samples. The kits typically come with pre-coated plates, detection reagents, calibration standards, wash buffers, and substrates, following a sandwich ELISA format that maximizes assay specificity and reproducibility. 

Applications:

• Cancer Research: NEIL enzymes play a role in counteracting oxidative DNA damage, a factor linked to cancer initiation and progression. Elevated or reduced NEIL activity can influence mutation rates and genomic instability, impacting cancer development. NEIL ELISA kits are used to study the expression and activity of NEIL enzymes in tumor samples, allowing researchers to investigate their role in cancer biology and assess the potential of targeting DNA repair pathways in anti-cancer therapies.
• Neurodegenerative Disease Studies: Oxidative stress and DNA damage are associated with neurodegenerative diseases such as Alzheimer’s, Parkinson’s, and Huntington’s diseases. NEIL ELISA kits help quantify the expression of NEIL enzymes in brain tissues or other relevant samples, enabling researchers to study how DNA repair capacity is affected in neurodegenerative conditions and potentially explore NEIL proteins as biomarkers for early detection or progression of these diseases.
• Aging and Cellular Senescence Research: NEIL enzymes contribute to genomic stability, a key factor in aging. Reduced DNA repair capacity over time can lead to accumulated mutations, contributing to cellular senescence and aging. NEIL ELISA kits are used in aging research to study the expression of NEIL proteins and their correlation with age-related cellular changes, helping to elucidate the mechanisms of aging and longevity.

NEIL ELISA Kits provide a reliable and quantitative approach to studying NEIL DNA glycosylases across various research areas. By enabling precise measurements of NEIL proteins, these kits support advances in understanding DNA repair mechanisms, exploring disease pathogenesis, and developing strategies to mitigate oxidative DNA damage in health and disease.