NGAL ELISA Kits
NGAL (Neutrophil Gelatinase-Associated Lipocalin) ELISA Kits are specialized immunoassays designed for the quantitative detection of NGAL protein in various biological samples. NGAL is a small glycoprotein involved in immune response and iron transport, and it serves as an early biomarker for acute kidney injury (AKI). Its levels rise rapidly in response to kidney injury, infection, inflammation, and certain cancers, making NGAL a valuable biomarker in nephrology, oncology, and infectious disease research.
Content of NGAL ELISA Kits
A standard NGAL ELISA Kit typically includes:
- Standards and Controls: A set of calibrated NGAL standards for generating a standard curve and positive and negative controls for assay validation.
- Substrate Solution: Chromogenic or chemiluminescent substrate that reacts with the enzyme to produce a measurable signal proportional to the NGAL concentration.
- Stop Solution: Stops the enzymatic reaction, allowing for accurate endpoint measurement.
- Wash Buffer and Sample Diluent: Solutions to optimize assay conditions, reducing background noise and enhancing specificity.
Application of NGAL ELISA Kits
NGAL ELISA Kits have significant applications in research and clinical settings, particularly in nephrology, oncology, and inflammation studies:
- Oncology Research: NGAL is overexpressed in several cancers, including breast, lung, and colorectal cancer. NGAL ELISA Kits are used to quantify NGAL levels in tumor samples or serum, supporting research into its role as a biomarker for cancer progression and metastasis.
- Inflammation and Infection Studies: NGAL is upregulated in response to infection and inflammation, especially in bacterial infections. Researchers use NGAL ELISA Kits to study immune response mechanisms and to evaluate NGAL as a potential biomarker for inflammatory and infectious diseases.
- Drug Development and Toxicology: NGAL is used as a biomarker in preclinical studies to assess nephrotoxicity of new drug compounds. NGAL ELISA Kits are valuable in drug development, allowing researchers to monitor kidney function in response to various therapeutic compounds.
The NGAL ELISA Kit is a versatile and powerful tool for researchers and clinicians in nephrology, oncology, and immunology, offering precise and sensitive quantification of NGAL for advancing understanding in kidney injury, inflammation, and cancer research.
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