NGF ELISA Kits
NGF ELISA kits are designed to detect and quantify NGF concentrations in various biological samples, including serum, plasma, cell culture supernatants, and tissue homogenates. These kits typically employ a sandwich ELISA format, where specific antibodies capture NGF, and detection is facilitated through enzyme-linked secondary antibodies, resulting in a measurable signal proportional to the NGF concentration.
Key Components:
- Pre-coated Microplate: Wells pre-coated with anti-NGF antibodies to capture the target protein.
- Standards and Controls: Known concentrations of NGF to generate a standard curve and validate assay performance.
- Detection Antibodies: Enzyme-conjugated antibodies specific to NGF for signal generation.
- Substrate Solution: Reacts with the enzyme to produce a detectable signal, typically colorimetric.
- Buffers and Wash Solutions: Ensure optimal reaction conditions and remove unbound substances.
Applications:
- Neuroscience Research: Investigating the role of NGF in neuronal development, survival, and function.
- Clinical Studies: Assessing NGF levels in patients with neurological disorders, such as Alzheimer's disease, Parkinson's disease, and peripheral neuropathies.
- Pharmaceutical Development: Evaluating the impact of therapeutic agents on NGF expression and function.
Examples of NGF ELISA Kits:
- Invitrogen™ Human NGF beta ELISA Kit: Quantifies human NGF in plasma, serum, and supernatant samples with a sensitivity of 14 pg/mL. Thermo Fisher Scientific
- R&D Systems Human beta-NGF DuoSet ELISA: Measures natural and recombinant human beta-NGF in cell culture supernates, serum, and plasma. Rndsystems
- Sigma-Aldrich Human β-NGF ELISA Kit: Suitable for serum, plasma, cell culture supernatant, and urine samples, with a sensitivity of 15 pg/mL. Sigma-Aldrich
- Biosensis NGF Rapid™ ELISA Kit: Allows quantification of human NGF in less than 3 hours in cell culture supernatants, serum, plasma (citrate), and brain extracts. Biosensis
By providing reliable and quantitative measurements of NGF, ELISA kits are indispensable tools in advancing our understanding of neurobiology and facilitating the diagnosis and management of neurological disorders.
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