NOX ELISA Kits

NOX (NADPH Oxidase) ELISA kits are immunoassays designed to detect and quantify NOX enzyme subunits or related proteins in biological samples such as serum, plasma, cell lysates, and tissue extracts. NADPH oxidases are a family of enzymes that play critical roles in generating reactive oxygen species (ROS) as part of cellular processes including immune response, cell signaling, and oxidative stress regulation. NOX enzymes are implicated in various pathological conditions, such as cardiovascular diseases, neurodegenerative disorders, and cancer.

Content: NOX ELISA kits are usually based on a sandwich ELISA format for high specificity and sensitivity. Typical kit components include:

• 96-well microplate pre-coated with a capture antibody specific to the NOX protein or subunit.
• Standards and controls for creating a standard curve for quantitative analysis.
• Biotinylated detection antibody, which binds to an epitope on the target NOX protein.
• Streptavidin-HRP enzyme conjugate to enhance detection through signal amplification.
• TMB substrate solution, which reacts with HRP to produce a colorimetric change.
• Stop solution to terminate the reaction.
• Wash buffers and assay diluents for consistent assay performance and reproducibility.

Applications: NOX ELISA kits are applied in various research and clinical contexts, such as:

• Oxidative Stress Studies: Measuring NOX activity or expression levels to investigate oxidative stress-related mechanisms in diseases.
• Cardiovascular Research: Studying the role of NOX enzymes in hypertension, atherosclerosis, and other cardiovascular conditions where ROS play a key role.
• Neuroscience: Evaluating NOX involvement in neurodegenerative disorders like Alzheimer’s disease and Parkinson’s disease, where oxidative damage is implicated.
• Cancer Research: Analyzing NOX expression and its role in tumor growth, metastasis, and cell survival through ROS-mediated signaling pathways.
• Inflammatory and Immune Response Studies: Investigating the contribution of NOX to immune cell function and its role in inflammation and host defense.

NOX ELISA kits are crucial tools for researchers studying the generation of reactive oxygen species and the involvement of NADPH oxidases in various biological processes and diseases. These kits enable accurate quantification of NOX subunits, facilitating a deeper understanding of their role in health and disease. Their application in oxidative stress research, cardiovascular studies, neurodegenerative diseases, and cancer provides essential insights into potential therapeutic targets and disease mechanisms.