NUDT ELISA Kits

NUDT (Nudix Hydrolase) ELISA kits are immunoassays designed for the quantitative detection of NUDT proteins in biological samples such as serum, plasma, and cell lysates. NUDT proteins are part of the Nudix hydrolase family, enzymes that hydrolyze nucleoside diphosphates linked to other moieties (e.g., ADP-ribose), playing essential roles in cellular metabolism, nucleotide regulation, and the maintenance of genomic stability. 

Content of NUDT ELISA Kits: NUDT ELISA kits typically include:

• A 96-well microplate pre-coated with capture antibodies specific to the targeted NUDT protein (e.g., NUDT1, also known as MTH1).
• Recombinant NUDT protein standards for generating a standard curve.
• Detection antibodies conjugated with an enzyme, such as HRP (Horseradish Peroxidase), for colorimetric signal development.
• Substrate solution (e.g., TMB – 3,3',5,5'-Tetramethylbenzidine) for signal visualization.
• Stop solution to terminate the enzymatic reaction.
• Sample and assay diluents, wash buffers, and a detailed instruction manual for assay preparation and execution.

Application of NUDT ELISA Kits: NUDT ELISA kits are employed in a variety of research and clinical applications, including:

• Cancer research: Measuring the expression of NUDT proteins, such as NUDT1 (MTH1), to understand their role in tumor growth and genomic stability. Overexpression of certain NUDT proteins has been linked to the protection of cancer cells from oxidative damage.
• Oxidative stress studies: Investigating the function of NUDT proteins in mitigating the effects of oxidative stress by hydrolyzing oxidized nucleotides and preventing their incorporation into DNA or RNA.
• DNA repair and genomic maintenance: Exploring how NUDT proteins contribute to the cellular response to DNA damage and support genomic integrity.
• Neurodegenerative disease research: Analyzing the potential role of NUDT proteins in protecting neurons from oxidative damage, relevant to diseases like Alzheimer’s and Parkinson’s.
• Pharmacological studies: Screening and evaluating inhibitors targeting specific NUDT proteins, as potential therapeutic agents for cancer treatment or managing oxidative stress-related conditions.
• Metabolic research: Understanding the broader implications of NUDT proteins in cellular nucleotide pools and metabolic regulation.

NUDT ELISA kits are valuable tools for researchers investigating cellular responses to oxidative stress, DNA repair mechanisms, and related diseases, providing critical insights into the roles of Nudix hydrolases in health and disease.