Overview of OR5 ELISA Kits

OR5 ELISA Kits are designed for the quantitative measurement of Olfactory Receptor 5 (OR5) proteins in various biological samples. OR5 proteins are part of the olfactory receptor family, involved in detecting odor molecules and playing a crucial role in the sense of smell.

Technical Content

Key Features:

• High Sensitivity: Capable of detecting low levels of OR5 proteins, ensuring precise quantification even in samples with low expression levels.
• Specificity: High specificity for OR5 proteins, minimizing cross-reactivity with other proteins.
• Wide Range of Applications: Suitable for use with tissue lysates, cell lysates, and other biological fluids.

Applications:

• Neuroscience Research: Used to study the expression and function of olfactory receptors in the olfactory system.
• Sensory Biology: Facilitates the investigation of odor detection mechanisms and olfactory signal transduction.
• Genetic Studies: Assists in understanding the genetic regulation of olfactory receptors and their roles in sensory perception.

Usage Protocol:

• Sample Preparation: Collect and prepare samples according to the kit instructions. Dilute if necessary.
• Assay Procedure: Add samples, standards, and controls to the wells of the ELISA plate pre-coated with OR5-specific antibodies.
• Incubation: Incubate the plate to allow binding of OR5 proteins to the antibodies.
• Detection: Add detection antibodies and enzyme substrate to generate a colorimetric signal proportional to the OR5 protein concentration.
• Analysis: Measure the absorbance using a microplate reader and calculate OR5 protein concentrations based on the standard curve.

Benefits:

• Precision: Provides reliable and reproducible results for the accurate quantification of OR5 proteins.
• Efficiency: Simplified protocol with ready-to-use reagents reduces assay time and labor.
• Versatility: Compatible with a variety of sample types, making it suitable for diverse research applications.