PAL ELISA Kits

Phenylalanine Ammonia-Lyase (PAL) is an enzyme pivotal in the phenylpropanoid pathway, catalyzing the deamination of L-phenylalanine to trans-cinnamic acid. This pathway is essential for the biosynthesis of various secondary metabolites, including flavonoids, lignin, and other phenolic compounds, which are crucial for plant development and defense mechanisms.

Key Features:

• Sensitivity and Specificity: Designed to detect low concentrations of PAL with high specificity, minimizing cross-reactivity with similar enzymes.
• Assay Range: Typically, these kits offer a detection range suitable for physiological levels of PAL, for example, 0.78 U/g tissue to 156 U/g tissue, with sensitivities around 0.78 U/g tissue.
• Sample Types: Validated for use with various sample matrices, including plant tissues, serum, plasma, and cell culture supernatants, providing versatility for different research needs.
• Assay Time: The total assay duration varies but generally ranges from 1 to 2 hours, facilitating efficient processing of multiple samples.

Applications:

• Plant Physiology and Biochemistry: PAL is a key enzyme in plant secondary metabolism. Measuring its activity is essential in studies related to plant growth, development, and response to environmental stresses.
• Agricultural Research: PAL activity is associated with plant defense mechanisms against pathogens. Quantifying PAL can help in breeding programs aimed at developing disease-resistant crop varieties.
• Pharmaceutical Research: PAL is involved in the biosynthesis of phenolic compounds with medicinal properties. Monitoring PAL levels can aid in optimizing the production of these compounds in medicinal plants.

By providing accurate quantification of PAL levels, these ELISA kits are invaluable tools in plant biology, agricultural research, and related fields, facilitating studies on plant metabolism, defense mechanisms, and the biosynthesis of phenolic compounds.