RFTN ELISA Kits

Raftlin (RFTN1) ELISA kits are specialized immunoassays designed for the quantitative measurement of Raftlin, a lipid raft linker protein, in various biological samples such as serum, plasma, tissue homogenates, and cell culture supernatants. Raftlin plays a crucial role in the formation and maintenance of lipid rafts, which are essential for signal transduction and membrane organization.

Technical Specifications:

• Assay Principle: These kits typically employ a sandwich ELISA format. A capture antibody specific to RFTN1 is pre-coated onto microplate wells. Samples or standards are added, allowing RFTN1 to bind to the capture antibody. A biotinylated detection antibody specific to a different epitope of RFTN1 is then introduced, forming a capture-detection antibody complex. Subsequent addition of streptavidin-HRP conjugate and a chromogenic substrate enables colorimetric detection, with the intensity of the color proportional to the RFTN1 concentration in the sample.
• Sensitivity and Range: The detection range and sensitivity vary among kits. For instance, the Human RFTN1 (Raftlin) QuickTest ELISA Kit from FineTest offers a detection range of 31.25–2000 pg/mL with a sensitivity of 18.75 pg/mL. FN Test
• Sample Volume: Typically, a sample volume of 50–100 µL is required per well, depending on the specific kit's protocol.

Applications:

• Immunology Research: Quantifying RFTN1 levels aids in understanding its role in immune cell signaling and function, particularly in T-cell receptor-mediated signaling.
• Cell Biology Studies: RFTN1 is involved in the formation and maintenance of lipid rafts, which are essential for various cellular processes, including signal transduction and membrane organization.
• Disease Mechanism Investigation: Altered RFTN1 expression has been associated with certain diseases, making it a potential biomarker for diagnostic or therapeutic research.

When selecting an RFTN1 ELISA kit, it is crucial to consider factors such as species reactivity, sample type compatibility, sensitivity, and detection range to ensure optimal performance for specific research applications.