SAA ELISA Kits

SAA (Serum Amyloid A) ELISA Kits are advanced immunoassays used for the quantitative measurement of serum amyloid A proteins in various biological samples such as serum, plasma, and tissue homogenates. SAA is an acute-phase protein produced predominantly by the liver in response to inflammatory stimuli. Its levels can rise dramatically during acute and chronic inflammatory conditions, making it a critical marker in clinical and research applications for monitoring inflammatory status and disease progression.

Content:

SAA ELISA Kits are built on the highly specific sandwich enzyme-linked immunosorbent assay format. The process involves several steps to ensure high sensitivity and specificity:

• Capture Phase: The 96-well plate is pre-coated with specific capture antibodies that bind to the SAA protein present in the sample.
• Detection Phase: A biotinylated secondary antibody, specific for a different epitope on the SAA protein, is added to form a sandwich complex with the captured SAA.
• Signal Development: Enzyme-conjugated streptavidin, typically linked to horseradish peroxidase (HRP), binds to the biotinylated detection antibody. A substrate solution is added to produce a colorimetric change.

Application of SAA ELISA Kits:

SAA ELISA Kits are critical in research and clinical settings to study inflammatory and autoimmune diseases, as well as in the context of metabolic and cardiovascular health. Specific applications include:

• Inflammation and Infection Monitoring: SAA levels are used to monitor the acute-phase response in various inflammatory conditions such as sepsis, infections, and chronic inflammatory diseases like rheumatoid arthritis.
• Cardiovascular Research: Elevated SAA levels have been associated with an increased risk of atherosclerosis and cardiovascular diseases. Researchers utilize these kits to study the role of SAA in the development and progression of cardiovascular disorders.
• Cancer Research: SAA has been implicated as a biomarker in certain cancers where chronic inflammation is a contributing factor. Measuring SAA can help assess tumor progression and patient response to therapy.

The high sensitivity and specificity of SAA ELISA Kits make them essential for accurate measurement of SAA levels, facilitating research into the pathophysiology of inflammation and aiding in the diagnosis and monitoring of inflammatory and metabolic conditions.