SDC ELISA Kits

SDC (Syndecan) ELISA Kits are specialized immunoassay systems used for the quantitative measurement of syndecan proteins in biological samples, such as serum, plasma, cell lysates, and tissue homogenates. Syndecans are a family of transmembrane heparan sulfate proteoglycans involved in a variety of cellular processes, including cell adhesion, migration, proliferation, and tissue repair. 

Content:

SDC ELISA Kits are designed using a sandwich-based enzyme-linked immunosorbent assay format to ensure high specificity and sensitivity. The assay typically includes the following steps:

• Capture Phase: The wells of the ELISA plate are pre-coated with capture antibodies that bind to specific SDC proteins.
• Sample Incubation: Samples containing SDC bind to the capture antibodies on the plate.
• Detection Phase: A biotinylated or enzyme-linked detection antibody binds to another epitope on the SDC protein, forming a sandwich complex.
• Quantification: The color intensity is measured using an ELISA plate reader at a specific wavelength, which is proportional to the concentration of SDC protein in the sample.

Application of SDC ELISA Kits:

SDC ELISA Kits are employed in various research and clinical applications to study the expression and role of syndecans in health and disease. Primary applications include:

• Cancer Research: Syndecans, especially SDC1, are involved in tumor progression, angiogenesis, and metastasis. Measuring SDC levels can help in assessing tumor growth and understanding mechanisms of cell invasion and adhesion.
• Wound Healing and Tissue Repair: Syndecans play a critical role in the wound healing process by mediating interactions with growth factors and the extracellular matrix. Quantifying SDC proteins helps researchers understand their involvement in tissue repair and regeneration.
• Inflammatory and Autoimmune Diseases: Changes in the expression of syndecans are associated with inflammatory responses. SDC ELISA Kits are used to evaluate their role as potential biomarkers for diseases such as rheumatoid arthritis and inflammatory bowel disease.

The high specificity and sensitivity of SDC ELISA Kits make them essential tools for accurately measuring syndecan protein concentrations, facilitating research into cellular processes and disease mechanisms involving cell signaling, adhesion, and migration.