The SERPINC (Serpin C) ELISA Kit is designed for the quantitative measurement of Serpin C (also known as Antithrombin III or ATIII) in biological samples. Serpin C is a crucial serine protease inhibitor that regulates blood clotting by inhibiting thrombin and other clotting factors. It plays an important role in maintaining hemostasis and preventing excessive clot formation.

Content of the Kit

• Pre-coated 96-Well Microplate:
  • Wells pre-coated with an antibody specific to Serpin C, allowing for capture of Serpin C from the samples.
• Standards:
  • Solutions containing known concentrations of recombinant or purified Serpin C, used to generate a standard curve for quantifying Serpin C levels in samples.
• Sample Diluent:
  • A buffer solution used to dilute biological samples to appropriate concentrations for accurate measurement.
• Detection Antibody:
  • An antibody specific to a different epitope of Serpin C, often conjugated to an enzyme such as HRP for detection.
• Enzyme Conjugate:
  • If the detection antibody is biotinylated, a streptavidin-HRP conjugate may be included for signal detection.

Procedure

• Coating:
  • Coat the wells of the microplate with an antibody specific to Serpin C. Incubate to allow binding and then wash away unbound antibody.
• Blocking:
  • Block any remaining uncoated surfaces in the wells with a blocking buffer to prevent non-specific binding.
• Addition of Standards and Samples:
  • Add standards and samples to the wells. The Serpin C in the samples will bind to the immobilized antibody.
• Incubation:
  • Incubate the plate to allow Serpin C to bind to the antibody.
• Addition of Detection Antibody:
  • Add a detection antibody specific to Serpin C, which binds to the captured Serpin C.

Performance Characteristics

• Sensitivity: Capable of detecting low levels of Serpin C, typically in the picogram per milliliter (pg/mL) range.
• Precision: Shows low intra-assay and inter-assay variability, with coefficients of variation (CV) generally below 10%.
• Linearity: Demonstrates linearity across the standard curve range, ensuring accurate quantification.
• Recovery: High recovery rates, usually above 85%, indicating reliable performance across different sample types.

Applications

• Coagulation Disorders: Measuring Serpin C levels to diagnose and monitor conditions like antithrombin deficiency.
• Thrombotic Diseases: Assessing Serpin C as a marker in thrombotic conditions and evaluating the effectiveness of anticoagulant therapies.
• Research: Studying the role of Serpin C in various physiological and pathological processes, including blood clotting and cardiovascular diseases.