Overview of SOCS ELISA Kits
SOCS ELISA Kits are designed for the quantitative measurement of Suppressor of Cytokine Signaling (SOCS) proteins in various biological samples. SOCS proteins are key regulators of cytokine signaling pathways, playing crucial roles in immune responses and cell signaling.
Technical Content
Key Features:
- High Sensitivity: Capable of detecting low levels of SOCS proteins, ensuring precise quantification even in samples with low expression levels.
- Specificity: High specificity for target SOCS proteins, minimizing cross-reactivity with other proteins.
- Broad Applicability: Suitable for use with serum, plasma, cell lysates, and other biological fluids.
Applications:
- Immunology Research: Used to study the regulation of cytokine signaling pathways and immune responses by SOCS proteins.
- Cancer Research: Facilitates the investigation of SOCS proteins' roles in tumor progression, immune evasion, and therapeutic resistance.
- Inflammation Studies: Assists in understanding the involvement of SOCS proteins in inflammatory diseases and conditions.
Usage Protocol:
- Sample Preparation: Collect and prepare samples according to the kit instructions. Dilute if necessary.
- Assay Procedure: Add samples, standards, and controls to the wells of the ELISA plate pre-coated with antibodies specific to the target SOCS protein.
- Incubation: Incubate the plate to allow binding of the SOCS proteins to the antibodies.
- Detection: Add detection antibodies and enzyme substrate to produce a colorimetric signal proportional to the SOCS protein concentration.
- Analysis: Measure the absorbance using a microplate reader and calculate SOCS protein concentrations based on the standard curve.
Benefits:
- Precision: Provides reliable and reproducible results for the accurate quantification of SOCS proteins.
- Efficiency: Simplified protocol with ready-to-use reagents reduces assay time and labor.
- Versatility: Compatible with a variety of sample types, making it suitable for diverse research applications.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|