Steroid Markers ELISA Kits

ELISA kits designed for steroid markers employ specific antibodies to detect and quantify steroid hormones in biological samples. These assays typically utilize a competitive binding format, where the target hormone in the sample competes with a labeled hormone for binding sites on a specific antibody. The resulting signal inversely correlates with the concentration of the hormone in the sample, allowing for precise quantification.

Key Components:

• Pre-coated Microplate: Wells coated with specific antibodies against the target steroid hormone.
• Standards and Controls: Known concentrations of the hormone to generate a standard curve and validate assay performance.
• Enzyme Conjugate: A hormone conjugated to an enzyme, facilitating detection.
• Substrate Solution: Reacts with the enzyme to produce a measurable signal, typically colorimetric or chemiluminescent.
• Buffers and Wash Solutions: Ensure optimal reaction conditions and remove unbound substances.

Applications:

• Clinical Diagnostics: Assessing hormone levels to diagnose endocrine disorders, reproductive health issues, and adrenal function abnormalities.
• Pharmaceutical Research: Evaluating the effects of drugs on hormone levels and endocrine function.
• Sports Medicine: Monitoring athletes for performance-enhancing steroid use.
• Environmental Studies: Investigating the impact of endocrine-disrupting chemicals on wildlife and human health.

Examples of Steroid Marker ELISA Kits:

• Cortisol ELISA Kits: Measure cortisol levels in serum, plasma, saliva, or urine. For instance, Neogen offers a Cortisol ELISA Kit suitable for various biological fluids. Neogen
• Testosterone ELISA Kits: Quantify testosterone concentrations, aiding in the assessment of gonadal function and related disorders.
• Estradiol ELISA Kits: Determine estradiol levels, important for evaluating ovarian function and menstrual irregularities.
• Progesterone ELISA Kits: Measure progesterone to monitor ovulation, assess luteal function, and support pregnancy evaluations.

By providing reliable and quantitative measurements of steroid hormones, ELISA kits are indispensable tools in advancing our understanding of endocrine function and facilitating the diagnosis and management of related disorders.