The TIRAP (Toll-interleukin-1 receptor (TIR) domain-containing adapter protein) ELISA Kit is designed to quantitatively measure the levels of TIRAP in biological samples. TIRAP is a key adapter protein involved in the Toll-like receptor (TLR) signaling pathway, which is crucial for the immune response and inflammation. The TIRAP ELISA Kit is used for research and diagnostic purposes to assess TIRAP levels in various contexts, including inflammation, immune response, and infectious diseases.

Content of the Kit

• Pre-coated 96-Well Microplate:
  • Wells pre-coated with an antibody specific to TIRAP, allowing for capture of TIRAP from the samples.
• Standards:
  • Solutions containing known concentrations of recombinant or purified TIRAP, used to generate a standard curve for quantifying TIRAP levels in samples.
• Sample Diluent:
  • A buffer solution used to dilute biological samples to appropriate concentrations for accurate measurement.
• Detection Antibody:
  • An antibody specific to a different epitope of TIRAP, often conjugated to an enzyme such as HRP for detection.
• Enzyme Conjugate:
  • If the detection antibody is biotinylated, a streptavidin-HRP conjugate may be included for signal detection.

Procedure

• Coating:
  • Coat the wells of the microplate with an antibody specific to TIRAP. Incubate to allow binding and then wash away unbound antibody.
• Blocking:
  • Block any remaining uncoated surfaces in the wells with a blocking buffer to prevent non-specific binding.
• Addition of Standards and Samples:
  • Add standards and samples to the wells. The TIRAP in the samples will bind to the immobilized antibody.
• Incubation:
  • Incubate the plate to allow TIRAP to bind to the antibody.
• Addition of Detection Antibody:
  • Add a detection antibody specific to TIRAP, which binds to the captured TIRAP.

Performance Characteristics

• Sensitivity: Capable of detecting low levels of TIRAP, typically in the picogram per milliliter (pg/mL) range.
• Precision: Shows low intra-assay and inter-assay variability, with coefficients of variation (CV) generally below 10%.
• Linearity: Demonstrates linearity across the standard curve range, ensuring accurate quantification.
• Recovery: High recovery rates, usually above 85%, indicating reliable performance across different sample types.

Applications

• Immune System Research: Study of TLR signaling pathways and their role in immune responses.
• Inflammatory Diseases: Assessing TIRAP levels in conditions involving chronic inflammation.
• Infectious Diseases: Monitoring TIRAP as a marker of response to infections.
• Cancer Research: Investigating the role of TIRAP in cancer development and progression.