TNFRSF10A ELISA Kits are designed for the detection and quantification of TNFRSF10A, also known as Death Receptor 4 (DR4) or TRAIL-R1. TNFRSF10A is a receptor in the TNF (Tumor Necrosis Factor) receptor superfamily that mediates apoptosis (programmed cell death) in response to TRAIL (TNF-related apoptosis-inducing ligand). This receptor plays a significant role in regulating cell death and is important in cancer research and the study of immune responses.

Content of a Standard TNFRSF10A ELISA Kit

• Microtiter Plate: Pre-coated with a capture antibody specific to TNFRSF10A.
• Standards: Recombinant TNFRSF10A protein at various concentrations to create a standard curve.
• Detection Antibody: Biotinylated or enzyme-conjugated anti-TNFRSF10A antibody for detecting the captured TNFRSF10A.
• Enzyme Conjugate: Typically streptavidin-HRP or another enzyme conjugate used to amplify the detection signal.
• Substrate Solution: Usually TMB (3,3',5,5'-Tetramethylbenzidine) or other substrates that react with HRP to produce a color change.
• Stop Solution: Often sulfuric acid or hydrochloric acid, which stops the enzyme reaction and stabilizes the color change.

Key Steps in the TNFRSF10A ELISA Procedure

• Sample/Standard Addition: Standards and samples are added to wells pre-coated with anti-TNFRSF10A antibody.
• Incubation: The plate is incubated to allow TNFRSF10A to bind to the capture antibody.
• Washing: Wells are washed to remove unbound proteins and reduce background noise.
• Detection Antibody Addition: A detection antibody specific to TNFRSF10A is added, binding to the captured TNFRSF10A.
• Washing: Excess detection antibody is removed by washing.
• Enzyme Conjugate Addition: Enzyme-conjugated secondary antibody is added, binding to the detection antibody.

Applications

• Cancer Research: Investigating the role of TNFRSF10A in cancer cell apoptosis and evaluating potential therapies that target DR4.
• Immunology: Studying the mechanisms of cell death and immune response regulation mediated by TNFRSF10A.
• Drug Development: Assessing drugs designed to modulate apoptosis through TNFRSF10A.

Important Considerations

• Sensitivity and Specificity: Ensure the ELISA kit is specific to TNFRSF10A to avoid cross-reactivity and provide accurate results.
• Sample Handling: Proper handling and preparation of samples are crucial to prevent degradation of TNFRSF10A and ensure accurate measurement.

TNFRSF10A ELISA kits are essential tools for researchers studying apoptosis and immune regulation. They provide critical insights into the role of TNFRSF10A in health and disease, contributing to advancements in cancer research and therapeutic development.