TNFRSF10B ELISA Kits are designed to detect and quantify TNFRSF10B, also known as Death Receptor 5 (DR5) or TRAIL-R2. TNFRSF10B is a receptor in the TNF (Tumor Necrosis Factor) receptor superfamily that is involved in mediating apoptosis (programmed cell death) through its interaction with TRAIL (TNF-related apoptosis-inducing ligand). This receptor plays a crucial role in regulating cell death and is implicated in cancer and various other diseases.

Content of a Standard TNFRSF10B ELISA Kit

• Microtiter Plate: Pre-coated with a capture antibody specific to TNFRSF10B.
• Standards: Recombinant TNFRSF10B protein at various concentrations to generate a standard curve.
• Detection Antibody: Biotinylated or enzyme-conjugated anti-TNFRSF10B antibody for specific detection of the captured TNFRSF10B.
• Enzyme Conjugate: Typically streptavidin-HRP or another enzyme conjugate used to amplify the signal.
• Substrate Solution: Usually TMB (3,3',5,5'-Tetramethylbenzidine) or other substrates that produce a color change upon reaction with HRP.
• Stop Solution: Often sulfuric acid or hydrochloric acid, which halts the enzymatic reaction and stabilizes the color change.

Key Steps in the TNFRSF10B ELISA Procedure

• Sample/Standard Addition: Standards and samples are added to wells pre-coated with anti-TNFRSF10B antibody.
• Incubation: The plate is incubated to allow TNFRSF10B to bind to the capture antibody.
• Washing: Wells are washed to remove unbound materials.
• Detection Antibody Addition: A detection antibody specific to TNFRSF10B is added, binding to the captured protein.
• Washing: Excess detection antibody is washed away.
• Enzyme Conjugate Addition: Enzyme-conjugated secondary antibody is added.

Applications

• Cancer Research: Studying the role of TNFRSF10B in cancer cell apoptosis and evaluating potential cancer therapies that target DR5.
• Cell Biology: Investigating the mechanisms of apoptosis and cell signaling pathways involving TNFRSF10B.
• Drug Development: Assessing the efficacy of drugs designed to modulate apoptosis through TNFRSF10B.

Important Considerations

• Sensitivity and Specificity: Ensure that the ELISA kit is highly sensitive and specific to TNFRSF10B to obtain accurate and reliable results.
• Sample Handling: Proper handling and preparation of samples are crucial to prevent degradation of TNFRSF10B and to ensure accurate quantification.

TNFRSF10B ELISA kits are valuable tools for research into apoptosis, cancer biology, and other areas where TNFRSF10B plays a critical role. They enable researchers to measure TNFRSF10B levels accurately, providing insights into its function and involvement in various diseases.