TNFRSF1 ELISA Kits

TNFRSF1A, also known as TNF Receptor 1 (TNFR1), is a receptor for Tumor Necrosis Factor-alpha (TNF-α), a key mediator in inflammation and apoptosis. TNFRSF1A plays a significant role in inflammatory signaling pathways and immune regulation. TNFRSF1A ELISA Kits are designed for the quantitative measurement of soluble TNFR1 levels in biological samples like serum, plasma, and cell culture supernatants. Due to its involvement in inflammatory responses, TNFR1 is frequently studied in autoimmune diseases, cancer, and infectious diseases.

Content of TNFRSF1A ELISA Kits

TNFRSF1A ELISA Kits typically include:

• Pre-coated ELISA Plate: Wells coated with an anti-TNFRSF1A antibody specific to TNFR1, ensuring high specificity and sensitivity for the detection of TNFRSF1A.
• Standards and Controls: A series of TNFRSF1A standards for constructing a standard curve, allowing for accurate quantification across a range of sample concentrations.
• Detection Antibody: A biotinylated or enzyme-linked antibody specific to TNFRSF1A, which binds to the captured TNFR1, amplifying the signal for enhanced sensitivity.
• Substrate Solution: Colorimetric substrates like TMB (3,3',5,5'-Tetramethylbenzidine) to generate a signal directly proportional to the TNFR1 concentration in the samples.
• Stop Solution and Wash Buffers: Components to terminate the reaction and wash away unbound materials, providing a clean and specific readout.

Application of TNFRSF1A ELISA Kits

TNFRSF1A ELISA Kits are widely used in:

• Cancer Research: TNFRSF1A signaling is involved in tumor growth, apoptosis, and immune escape mechanisms, particularly in cancers such as breast and colorectal cancer. TNFR1 measurement provides insights into tumor microenvironment and immune interactions, making it useful for biomarker research and therapeutic monitoring.
• Cardiovascular and Metabolic Disease Studies: TNFRSF1A is implicated in the inflammatory processes underlying cardiovascular diseases and metabolic syndrome. Elevated TNFR1 levels are associated with chronic inflammation in conditions like atherosclerosis and type 2 diabetes, making TNFR1 a valuable biomarker in these diseases.
• Infectious Disease Research: During infections, TNFRSF1A mediates inflammatory responses. Quantifying TNFR1 levels aids in understanding the immune response in infectious diseases and evaluating potential targets for anti-inflammatory treatments.

Overall, TNFRSF1A ELISA Kits provide reliable and quantitative measurements, essential for advancing research in inflammation, immune modulation, and disease pathology across a wide range of clinical and biomedical applications.