TNFRSF1A, also known as Tumor Necrosis Factor Receptor Superfamily Member 1A (TNFRSF1A) or TNF Receptor 1 (TNFR1), is a receptor for Tumor Necrosis Factor-alpha (TNF-α), which is a key cytokine involved in inflammation and immune responses. TNFRSF1A is involved in various cellular processes, including apoptosis, inflammation, and immune regulation. ELISA kits for TNFRSF1A are designed to quantify its levels in biological samples, such as serum, plasma, and cell culture supernatants.

Overview of TNFRSF1A ELISA Kits

• Purpose: To detect and quantify TNFRSF1A (TNF Receptor 1) levels in biological samples. These measurements are useful for studying inflammatory responses, autoimmune diseases, and various conditions involving TNF signaling.
• Assay Principle: Typically a sandwich ELISA. The assay involves capturing TNFRSF1A with a specific antibody immobilized on a microtiter plate, followed by detection with a secondary antibody conjugated to an enzyme (usually HRP). The enzyme-substrate reaction produces a color change that is proportional to the amount of TNFRSF1A present in the sample.
• Sensitivity: High sensitivity, often in the picogram per milliliter (pg/mL) range, suitable for detecting low levels of TNFRSF1A.
• Specificity: Highly specific to TNFRSF1A, with minimal cross-reactivity with other receptors or proteins.
• Sample Volume: Typically requires 50-100 µL of sample per well.

Content of a Standard TNFRSF1A ELISA Kit

• Microtiter Plate: Pre-coated with an anti-TNFRSF1A antibody.
• Standards: Recombinant TNFRSF1A provided at various concentrations to create a standard curve.
• Detection Antibody: Biotinylated or enzyme-conjugated anti-TNFRSF1A antibody.
• Enzyme Conjugate: Typically streptavidin-HRP, used for signal amplification.
• Substrate Solution: Usually TMB (3,3',5,5'-Tetramethylbenzidine), which produces a color change upon reaction with HRP.
• Stop Solution: Often a sulfuric acid solution that stops the enzyme reaction and stabilizes the color change.

Key Steps in the Assay Procedure

• Sample/Standard Addition: Samples or standards are added to wells pre-coated with an anti-TNFRSF1A antibody.
• Incubation: The plate is incubated, allowing TNFRSF1A to bind to the capture antibody.
• Washing: Wells are washed to remove unbound components.
• Detection Antibody Addition: The detection antibody specific to TNFRSF1A is added, binding to the captured receptor.
• Washing: Excess detection antibody is removed by washing.
• Enzyme Conjugate Addition: Enzyme-conjugated secondary antibody binds to the detection antibody.

Applications

• Research: Studying the role of TNFRSF1A in inflammation, apoptosis, and immune regulation, and its involvement in diseases such as rheumatoid arthritis, Crohn’s disease, and other autoimmune disorders.
• Clinical Diagnostics: Measuring TNFRSF1A levels can provide insights into disease mechanisms and potentially guide therapeutic interventions.

TNFRSF1A ELISA kits are important tools for understanding the role of TNFR1 in various biological processes and diseases, helping researchers and clinicians assess TNFRSF1A levels to better understand and manage conditions related to TNF signaling.