Transthyretin ELISA Kits

Transthyretin (TTR), also known as prealbumin, is a transport protein primarily synthesized in the liver and choroid plexus. It plays a crucial role in the transport of thyroxine (T4) and retinol-binding protein bound to retinol (vitamin A). Abnormal levels of TTR are associated with various clinical conditions, including malnutrition, liver dysfunction, and amyloid diseases such as familial amyloid polyneuropathy and senile systemic amyloidosis.

Key Features:

• Sensitivity and Specificity: Designed to detect low concentrations of TTR with high specificity, minimizing cross-reactivity with similar proteins.
• Assay Range: Typically, these kits offer a detection range suitable for physiological and pathological levels of TTR. For example, the Human TTR ELISA Kit from Assay Genie has a detection range of 0.312–20 ng/mL with a sensitivity of 0.188 ng/mL. Assay Genie
• Sample Types: Validated for use with various sample matrices, including serum, plasma, cerebrospinal fluid (CSF), tissue homogenates, and cell culture supernatants, providing versatility for different research needs.
• Assay Time: The total assay duration varies but generally ranges from 1 to 5 hours, facilitating efficient processing of multiple samples.

Applications:

• Clinical Diagnostics: TTR levels are used as a biomarker for nutritional status, particularly in assessing protein-energy malnutrition. Low TTR levels may indicate malnutrition or liver dysfunction, while elevated levels can be associated with inflammation or renal dysfunction.
• Amyloidosis Research: Mutations in the TTR gene can lead to amyloid fibril formation, resulting in conditions such as familial amyloid polyneuropathy and senile systemic amyloidosis. TTR ELISA kits are utilized to study TTR levels and their role in amyloid diseases.
• Pharmacological Research: In drug development, TTR levels serve as biomarkers to evaluate the efficacy of therapeutic agents targeting TTR-related amyloidosis. Monitoring TTR can help determine the impact of therapeutic interventions aimed at stabilizing TTR and preventing amyloid fibril formation.

By providing accurate quantification of TTR levels, these ELISA kits are invaluable tools in clinical diagnostics and biomedical research, facilitating studies on nutritional status, amyloid diseases, and the efficacy of therapeutic interventions.