Transthyretin (TTR) ELISA Kits are used to measure the concentration of Transthyretin in biological samples. Transthyretin is a protein that transports thyroid hormones and retinol (vitamin A) in the bloodstream and cerebrospinal fluid. It is also known for its role in various diseases, including amyloidosis, where it can misfold and aggregate into amyloid fibrils.
Content of the Kit
- Pre-coated 96-Well Microplate:
- Wells pre-coated with an antibody specific to Transthyretin, allowing for the capture of TTR from the samples.
- Standards:
- Solutions containing known concentrations of recombinant or purified Transthyretin, used to generate a standard curve for quantifying TTR levels in samples.
- Sample Diluent:
- A buffer solution used to dilute biological samples to appropriate concentrations for accurate measurement.
- Detection Antibody:
- An antibody specific to a different epitope of Transthyretin, often conjugated to an enzyme such as HRP for detection.
- Enzyme Conjugate:
- If the detection antibody is biotinylated, a streptavidin-HRP conjugate may be included for signal detection.
Procedure
- Coating:
- Coat the wells of the microplate with an antibody specific to Transthyretin. Incubate to allow binding and then wash away unbound antibody.
- Blocking:
- Block any remaining uncoated surfaces in the wells with a blocking buffer to prevent non-specific binding.
- Addition of Standards and Samples:
- Add standards and samples to the wells. The TTR in the samples will bind to the immobilized antibody.
- Incubation:
- Incubate the plate to allow Transthyretin to bind to the antibody.
- Addition of Detection Antibody:
- Add a detection antibody specific to Transthyretin, which binds to the captured TTR.
Performance Characteristics
- Sensitivity: Capable of detecting low levels of Transthyretin, typically in the picogram per milliliter (pg/mL) range.
- Precision: Shows low intra-assay and inter-assay variability, with coefficients of variation (CV) generally below 10%.
- Linearity: Demonstrates linearity across the standard curve range, ensuring accurate quantification.
- Recovery: High recovery rates, usually above 85%, indicating reliable performance across different sample types.
Applications
- Amyloidosis Research: Measuring TTR levels to understand its role in amyloidosis and other protein misfolding diseases.
- Thyroid Function Studies: Assessing TTR as a transporter of thyroid hormones and its implications in thyroid disorders.
- Clinical Diagnostics: Evaluating TTR levels in various diseases to assess its potential as a biomarker.
- Metabolic Research: Investigating TTR’s role in vitamin A transport and metabolism.
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