The TREM ELISA Kit is a specialized assay used to measure the concentration of Triggering Receptor Expressed on Myeloid Cells (TREM) proteins in biological samples. TREM proteins are important in various immune responses and are involved in inflammatory diseases, making their quantification crucial for research and diagnostic purposes.
Content of TREM ELISA Kit
- Microplate: A 96-well plate coated with a capture antibody specific to the TREM protein.
- Standards: Known concentrations of recombinant TREM protein to create a standard curve for quantification.
- Detection Antibody: A biotinylated antibody that specifically binds to the TREM protein.
- Enzyme Conjugate: Streptavidin-HRP (Horseradish Peroxidase) that binds to the biotinylated detection antibody.
- Substrate Solution: TMB (3,3',5,5'-Tetramethylbenzidine) substrate for the HRP enzyme to produce a colorimetric signal.
- Stop Solution: Usually sulfuric or phosphoric acid to stop the enzyme-substrate reaction.
- Sample Diluent: Buffer used to dilute samples to appropriate concentrations.
- Wash Buffer: Buffer used for washing wells between steps to remove unbound substances.
- Instruction Manual: Detailed instructions for performing the assay, including sample preparation, assay procedure, and data analysis.
Technical Summary
- Sensitivity: The kit is designed to detect low concentrations of TREM protein with high sensitivity.
- Specificity: High specificity due to the use of specific capture and detection antibodies, reducing cross-reactivity with other proteins.
- Assay Range: The range of TREM concentrations that can be accurately quantified, typically provided in the manual.
- Sample Types: Compatible with various biological samples such as serum, plasma, cell culture supernatants, and tissue homogenates.
- Assay Time: Total time required to complete the assay, including incubation steps and color development (usually around 4-5 hours).
- Storage Conditions: Specific conditions for storing the kit components to maintain their stability and performance, typically at 2-8°C.
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