The AffiELISA® Human AFF1 Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to AF4/FMR2 Family, Member 1 (AFF1) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to AF4/FMR2 Family, Member 1 (AFF1) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain AF4/FMR2 Family, Member 1 (AFF1) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of AF4/FMR2 Family, Member 1 (AFF1) in the samples can be determined.
AffiELISA® Human AFF1 Kit
AF4/FMR2 Family, Member 1
AF-4 - AF4 - AF4-MLL - MLL/AF4 - MLLT2 - PBM1 - Myeloid/Lymphoid Or Mixed-Lineage Leukemia - Translocated To 2 - Pre-B-Cell Monocytic Leukemia Partner 1
Human
Sandwich ELISA
Tissue homogenates, cell lysates and other biological fluids.
Signal transduction.